中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 海洋生物技术研发中心
Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye

文献类型：期刊论文

作者Feng Jinhua1,2; Yu Huahua1; Li Cuiping1,2; Xing Ronge1; Liu Song1; Wang Lin1,2; Cai Shengbao1,2; Li Pengcheng1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2009-11-01
卷号27期号:4页码:869-874
关键词Nematocyst Jellyfish Venom Rhopilema Esculentum Hemolytic Activity
ISSN号0254-4059
DOI10.1007/s00343-009-9225-9
文献子类Article
英文摘要The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 A mu g/mL.; The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 A mu g/mL.
学科主题Limnology ; Oceanography
URL标识查看原文
语种英语
WOS记录号WOS:000272422300023
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3471]  
专题海洋研究所_海洋生物技术研发中心
海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋环境工程技术研究发展中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Feng Jinhua,Yu Huahua,Li Cuiping,et al. Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(4):869-874.
APA Feng Jinhua.,Yu Huahua.,Li Cuiping.,Xing Ronge.,Liu Song.,...&Li Pengcheng.(2009).Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(4),869-874.
MLA Feng Jinhua,et al."Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.4(2009):869-874.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。