The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites
文献类型：期刊论文
| 作者 | Chen, Zhongke; Zhang, Hongwei; Yang, Hui ; Huang, Xiangwei; Zhang, Xiaohui; Zhang, Peijun
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 147期号:3页码:460-465 |
| 关键词 | Amphioxus Development Notochord Somitogenesis Amphitctp |
| ISSN号 | 1096-4959 |
| DOI | 10.1016/j.cbpb.2007.02.012 |
| 文献子类 | Article |
| 英文摘要 | The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved.; The translationally controlled tumor protein (TCTP) is highly conserved and has been widely found in eukaryotic organisms. Here, we report the phylogenetic analysis and developmental expression of AmphiTCTP, a TCTP homologous gene in cephalochordate amphioxus. Phylogenetic analysis indicates that the putative protein of AmphiTCTP is close to its vertebrate orthologs. The mRNA of AmphiTCTP is found in fertilized eggs, early cleavage embryo and most of the early developmental stages by in situ hybridization and RT-PCR, but its expression is not detectable from late cleavage stage to mid-gastrula. The expression of AmphiTCTP in zygotes and early cleavage stages shows that AmphiTCTP may be a maternal gene. From the early neurula stage onward, AmphiTCTP transcript is localized in the presumptive notochord, presomitic mesoderm, and nascent somites. However, its expression is gradually down-regulated after the notochord and somites have been formed. The expression pattern of AmphiTCTP thus coincides with the differentiation of the notochord and somites, this suggests that AmphiTCTP may not be a housekeeping gene and may play an important role in mesoderm development. (c) 2007 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Zoology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000247555900013 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5523]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Univ, Life Sci Coll, Key Lab Expt Teratol Minist Educ, Jinan 250012, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhongke,Zhang, Hongwei,Yang, Hui,et al. The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(3):460-465. |
| APA | Chen, Zhongke,Zhang, Hongwei,Yang, Hui,Huang, Xiangwei,Zhang, Xiaohui,&Zhang, Peijun.(2007).The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(3),460-465. |
| MLA | Chen, Zhongke,et al."The expression of AmphiTCTP, a TCTP orthologous gene in amphioxus related to the development of notochord and somites".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.3(2007):460-465. |
入库方式： OAI收割
来源：海洋研究所
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