Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii
文献类型:期刊论文
作者 | Chi, Xiaoyuan1,2; Zhang, Xiaowen1,2; Guan, Xiangyu3; Ding, Ling1,2; Li, Youxun1,2; Wang, Mingqing3; Lin, Hanzhi1,2; Qin, Song1 |
刊名 | JOURNAL OF MICROBIOLOGY |
出版日期 | 2008-04-01 |
卷号 | 46期号:2页码:189-201 |
ISSN号 | 1225-8873 |
关键词 | Fatty Acid Desaturase Eukaryotic Photosynthetic Microalgae Chlamydomonas Reinhardtii |
DOI | 10.1007/s12275-007-0223-3 |
文献子类 | Article |
英文摘要 | Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Delta 12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).; Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Delta 12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae). |
学科主题 | Microbiology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000255438700010 |
公开日期 | 2010-12-24 |
源URL | [http://ir.qdio.ac.cn/handle/337002/5527] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Fac Life Sci & Technol, Qingdao 266003, Peoples R China |
推荐引用方式 GB/T 7714 | Chi, Xiaoyuan,Zhang, Xiaowen,Guan, Xiangyu,et al. Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii[J]. JOURNAL OF MICROBIOLOGY,2008,46(2):189-201. |
APA | Chi, Xiaoyuan.,Zhang, Xiaowen.,Guan, Xiangyu.,Ding, Ling.,Li, Youxun.,...&Qin, Song.(2008).Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii.JOURNAL OF MICROBIOLOGY,46(2),189-201. |
MLA | Chi, Xiaoyuan,et al."Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii".JOURNAL OF MICROBIOLOGY 46.2(2008):189-201. |
入库方式: OAI收割
来源:海洋研究所
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