Study on early-stage development of conchospore in Porphyra yezoensis Ueda
文献类型：期刊论文
| 作者 | Fan Xiaolei1,2; Wang Guangce1,3; Li Demao1,2; Xu Pu4; Shen Songdong5 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2008-06-10 |
| 卷号 | 278期号:1-4页码:143-149 |
| 关键词 | Attachment Cell Wall Formation Conchospore Light Effects Porphyra Yezoensis |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2008.02.037 |
| 文献子类 | Article |
| 英文摘要 | Porphyra yezoensis Ueda (Rhodophyta) is a seaweed of economic importance with a typical dimorphic life cycle consisting of a leafy gametophyte and a filamentous sporophyte. Recently, it has been recognized as a model system for fundamental and applied studies in marine biological sciences. Conchospore, a major spore linking the two distinct multicellular phases in the life cycle, is most widely used in the breeding of P. yezoensis. In this paper, the early-stage development of conchospore, including the attachment and the cell wall formation, was studied with fluorescent reagents staining and Scanning Electron Microscopy detection. Results displayed: (I) the cell wall began to be generated after culturing for 4 h in the attached conchospores; (2) the initially released conchospores were plastids with some filmy, amorphous substance on the surface, and they attached to the fibers firmly via the actively secreted mucilaginous substances after their touch to the fibers; (3) cellulase and pectolase prohibited the attachment of conchospores in the different ways; and (4) only attached conchospores generated cell walls and developed normally, while the suspending ones could not. It indicated that the cellulose played crucial roles in the permanent attachment as the pectin did in the initial attachment. The conchospore attachment seemed to trigger the cell wall formation and the further development. Affects of light on the development of conchospores were also discussed. The results showed that high intensity (200 mu mol.m(-2).s(-1)) and long-wave (>= 580 nm) light facilitated the division rate of conchospores. (C) 2008 Elsevier B.V. All rights reserved.; Porphyra yezoensis Ueda (Rhodophyta) is a seaweed of economic importance with a typical dimorphic life cycle consisting of a leafy gametophyte and a filamentous sporophyte. Recently, it has been recognized as a model system for fundamental and applied studies in marine biological sciences. Conchospore, a major spore linking the two distinct multicellular phases in the life cycle, is most widely used in the breeding of P. yezoensis. In this paper, the early-stage development of conchospore, including the attachment and the cell wall formation, was studied with fluorescent reagents staining and Scanning Electron Microscopy detection. Results displayed: (I) the cell wall began to be generated after culturing for 4 h in the attached conchospores; (2) the initially released conchospores were plastids with some filmy, amorphous substance on the surface, and they attached to the fibers firmly via the actively secreted mucilaginous substances after their touch to the fibers; (3) cellulase and pectolase prohibited the attachment of conchospores in the different ways; and (4) only attached conchospores generated cell walls and developed normally, while the suspending ones could not. It indicated that the cellulose played crucial roles in the permanent attachment as the pectin did in the initial attachment. The conchospore attachment seemed to trigger the cell wall formation and the further development. Affects of light on the development of conchospores were also discussed. The results showed that high intensity (200 mu mol.m(-2).s(-1)) and long-wave (>= 580 nm) light facilitated the division rate of conchospores. (C) 2008 Elsevier B.V. All rights reserved. |
| 学科主题 | Fisheries ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000257004800021 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5541]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 4.Changshu Inst Technol, Changshu 215500, Peoples R China 5.Soochow Univ, Sch Life Sci, Suzhou 215006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan Xiaolei,Wang Guangce,Li Demao,et al. Study on early-stage development of conchospore in Porphyra yezoensis Ueda[J]. AQUACULTURE,2008,278(1-4):143-149. |
| APA | Fan Xiaolei,Wang Guangce,Li Demao,Xu Pu,&Shen Songdong.(2008).Study on early-stage development of conchospore in Porphyra yezoensis Ueda.AQUACULTURE,278(1-4),143-149. |
| MLA | Fan Xiaolei,et al."Study on early-stage development of conchospore in Porphyra yezoensis Ueda".AQUACULTURE 278.1-4(2008):143-149. |
入库方式： OAI收割
来源：海洋研究所
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