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Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes

文献类型：期刊论文

作者Fu, Wandong1,2; Yao, Jianting1,2; Wang, Xiuliang1; Liu, Fuli1,2; Chong, Zhuo1,2; Duan, Delin1
刊名PLANT MOLECULAR BIOLOGY REPORTER
出版日期2008-06-01
卷号26期号:2页码:114-120
关键词Brown Alga Laminaria Japonica Plastid Dna Ultracentrifugation
ISSN号0735-9640
DOI10.1007/s11105-008-0028-5
文献子类Article
英文摘要Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.; Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.
学科主题Biochemical Research Methods ; Plant Sciences
URL标识查看原文
语种英语
WOS记录号WOS:000259962500004
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5549]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Fu, Wandong,Yao, Jianting,Wang, Xiuliang,et al. Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes[J]. PLANT MOLECULAR BIOLOGY REPORTER,2008,26(2):114-120.
APA Fu, Wandong,Yao, Jianting,Wang, Xiuliang,Liu, Fuli,Chong, Zhuo,&Duan, Delin.(2008).Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes.PLANT MOLECULAR BIOLOGY REPORTER,26(2),114-120.
MLA Fu, Wandong,et al."Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes".PLANT MOLECULAR BIOLOGY REPORTER 26.2(2008):114-120.

入库方式： OAI收割

来源：海洋研究所

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