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Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis

文献类型：期刊论文

作者Luana, Wei; Li, Fuhua; Wang, Bing; Zhang, Xiaojun; Liu, Yichen; Xiang, Jianhai
刊名COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
出版日期2007-07-01
卷号147期号:3页码:482-491
关键词Calreticulin Cloning Expression Fenneropenaeus Chinensis Heat Shock Molting Stage Wssv Challenge
ISSN号1096-4959
DOI10.1016/j.cbpb.2007.03.001
文献子类Article
英文摘要Calreticulin (CRT), as an endoplasmic reticulum luminal resident protein, plays important roles in Ca2+ homeostasis and molecular chaperoning. CRT on the surface of the cell can modulate cell adhesion, phagocytosis and integrin-dependent Ca2+ signaling. The full length cDNA of calreticulin (FcCRT) was cloned from Chinese shrimp Fenneropenaeus chinensis. It consists of 1672 by with an open reading frame of 1221 bp, encoding 406 amino acids. This is the first reported cDNA sequence of calreticulin in Crustacea. The deduced amino acid sequence of FcCRT showed high identity with those of Bombyx mori (88%), Drosophila melanogaster (83%), Mus musculus (82%) and Homo sapiens (82%). Highest expression of FcCRT was detected in ovary by Northern blot and in situ hybridization. Different mRNA levels of FcCRT were detected at various molting stages. Expression of FcCRT was induced significantly after 3 h of heat shock treatment, reached the maximum at 4 h and dropped after that. Differential expression profiles of FcCRT were observed in hepatopancreas and haemocytes when shrimp were challenged by white spot syndrome virus (WSSV). From the above results, we inferred that FcCRT might play important roles in Ca2+ homeostasis, chaperoning and immune function in shrimp. (c) 2007 Elsevier Inc. All rights reserved.; Calreticulin (CRT), as an endoplasmic reticulum luminal resident protein, plays important roles in Ca2+ homeostasis and molecular chaperoning. CRT on the surface of the cell can modulate cell adhesion, phagocytosis and integrin-dependent Ca2+ signaling. The full length cDNA of calreticulin (FcCRT) was cloned from Chinese shrimp Fenneropenaeus chinensis. It consists of 1672 by with an open reading frame of 1221 bp, encoding 406 amino acids. This is the first reported cDNA sequence of calreticulin in Crustacea. The deduced amino acid sequence of FcCRT showed high identity with those of Bombyx mori (88%), Drosophila melanogaster (83%), Mus musculus (82%) and Homo sapiens (82%). Highest expression of FcCRT was detected in ovary by Northern blot and in situ hybridization. Different mRNA levels of FcCRT were detected at various molting stages. Expression of FcCRT was induced significantly after 3 h of heat shock treatment, reached the maximum at 4 h and dropped after that. Differential expression profiles of FcCRT were observed in hepatopancreas and haemocytes when shrimp were challenged by white spot syndrome virus (WSSV). From the above results, we inferred that FcCRT might play important roles in Ca2+ homeostasis, chaperoning and immune function in shrimp. (c) 2007 Elsevier Inc. All rights reserved.
学科主题Biochemistry & Molecular Biology ; Zoology
URL标识查看原文
语种英语
WOS记录号WOS:000247555900016
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5611]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Luana, Wei,Li, Fuhua,Wang, Bing,et al. Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(3):482-491.
APA Luana, Wei,Li, Fuhua,Wang, Bing,Zhang, Xiaojun,Liu, Yichen,&Xiang, Jianhai.(2007).Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(3),482-491.
MLA Luana, Wei,et al."Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.3(2007):482-491.

入库方式： OAI收割

来源：海洋研究所

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