Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01
文献类型：期刊论文
| 作者 | Sun, Yuying; Han, Baoqin; Liu, Wanshun; Zhang, Jiquan; Gao, Xingshuang |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 98期号:8页码:1548-1553 |
| 关键词 | Chitosanase Induction Colloidal Chitosan Statistical Optimization Plackett-burman Response Surface Methodology |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2006.07.020 |
| 文献子类 | Article |
| 英文摘要 | The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacteritan sp. OU01 in shake-flask. A significant influence of (NH(4))(2)SO(4), MgSO(4)center dot 7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0 g/L (NH(4))(2)SO(4), 1.3 g/L MgSO(4) and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6 U/mL to 118 U/mL. (c) 2006 Elsevier Ltd. All rights reserved.; The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacteritan sp. OU01 in shake-flask. A significant influence of (NH4)(2)SO4, MgSO4 center dot 7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0 g/L (NH4)(2)SO4, 1.3 g/L MgSO4 and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6 U/mL to 118 U/mL. (c) 2006 Elsevier Ltd. All rights reserved. |
| 学科主题 | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000244775100006 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5649]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuying,Han, Baoqin,Liu, Wanshun,et al. Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01[J]. BIORESOURCE TECHNOLOGY,2007,98(8):1548-1553. |
| APA | Sun, Yuying,Han, Baoqin,Liu, Wanshun,Zhang, Jiquan,&Gao, Xingshuang.(2007).Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01.BIORESOURCE TECHNOLOGY,98(8),1548-1553. |
| MLA | Sun, Yuying,et al."Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01".BIORESOURCE TECHNOLOGY 98.8(2007):1548-1553. |
入库方式： OAI收割
来源：海洋研究所
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