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Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

文献类型：期刊论文

作者Wang, Bing1; Li, Fuhua1; Luan, Wei1,2; Xie, Yusu1,2; Zhang, Chengsong1,2; Luo, Zhan1,2; Gui, Lang1,2; Yan, Hui1,2; Xiang, Jianhai1
刊名MARINE BIOTECHNOLOGY
出版日期2008-12-01
卷号10期号:6页码:664-675
关键词Wssv Vibrio Cdna Microarray Fenneropenaeus Chinensis
ISSN号1436-2228
DOI10.1007/s10126-008-9105-x
文献子类Article
英文摘要Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.; Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.
学科主题Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识查看原文
语种英语
WOS记录号WOS:000260838600005
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5661]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Bing,Li, Fuhua,Luan, Wei,et al. Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio[J]. MARINE BIOTECHNOLOGY,2008,10(6):664-675.
APA Wang, Bing.,Li, Fuhua.,Luan, Wei.,Xie, Yusu.,Zhang, Chengsong.,...&Xiang, Jianhai.(2008).Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio.MARINE BIOTECHNOLOGY,10(6),664-675.
MLA Wang, Bing,et al."Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio".MARINE BIOTECHNOLOGY 10.6(2008):664-675.

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来源：海洋研究所

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