Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain
文献类型：期刊论文
| 作者 | Wang, Fang1,2; Cheng, Shuang1,2; Sun, Kun1,2; Sun, Li1 |
| 刊名 | JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 46期号:3页码:350-355 |
| 关键词 | Edwardsiella Tarda Fur Autoregulation Virulence Mutation |
| ISSN号 | 1225-8873 |
| DOI | 10.1007/s12275-008-0038-x |
| 文献子类 | Article |
| 英文摘要 | The gene encoding the Edwardsiella tarda ferric uptake regulator (Fur(Et)) was cloned from a pathogenic E. tarda strain isolated from diseased fish. Fur(Et) shares 90% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and was able to complement the mutant phenotype of a fur(Ec)-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated Fur(Et) whereas E112K mutation resulted in a superactive Fur(Et) variant. Fur(Et) negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.; The gene encoding the Edwardsiella tarda ferric uptake regulator (Fur(Et)) was cloned from a pathogenic E. tarda strain isolated from diseased fish. Fur(Et) shares 90% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and was able to complement the mutant phenotype of a fur(Ec)-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated Fur(Et) whereas E112K mutation resulted in a superactive Fur(Et) variant. Fur(Et) negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000257240800017 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5673]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fang,Cheng, Shuang,Sun, Kun,et al. Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain[J]. JOURNAL OF MICROBIOLOGY,2008,46(3):350-355. |
| APA | Wang, Fang,Cheng, Shuang,Sun, Kun,&Sun, Li.(2008).Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain.JOURNAL OF MICROBIOLOGY,46(3),350-355. |
| MLA | Wang, Fang,et al."Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain".JOURNAL OF MICROBIOLOGY 46.3(2008):350-355. |
入库方式： OAI收割
来源：海洋研究所
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