N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro
文献类型：期刊论文
| 作者 | Wang, Zheng; Zheng, Lanhong; Yang, Shaoli; Niu, Rongli; Chu, Edward; Lin, Xiukun |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007-05-25 |
| 卷号 | 357期号:1页码:26-31 |
| 关键词 | N-acetylchitooligosaccharide Chitooligosaccharide Human Umbilical Vein Endothelial Cells Zebrafish Antiangiogenesis |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.03.094 |
| 文献子类 | Article |
| 英文摘要 | N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs. (c) 2007 Published by Elsevier Inc.; N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs. (c) 2007 Published by Elsevier Inc. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000246028800005 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5679]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Yale Univ, Sch Med, Ctr Canc, New Haven, CT 06511 USA |
| 推荐引用方式 GB/T 7714 | Wang, Zheng,Zheng, Lanhong,Yang, Shaoli,et al. N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,357(1):26-31. |
| APA | Wang, Zheng,Zheng, Lanhong,Yang, Shaoli,Niu, Rongli,Chu, Edward,&Lin, Xiukun.(2007).N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,357(1),26-31. |
| MLA | Wang, Zheng,et al."N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 357.1(2007):26-31. |
入库方式： OAI收割
来源：海洋研究所
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