Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm
文献类型：期刊论文
| 作者 | Xu, Jian-He1,3; You, Feng1; Sun, Wei1,2; Yan, Bin-Lun3; Zhang, Pei-Jun1; Jing, Bi-Xiang3 |
| 刊名 | AQUACULTURE INTERNATIONAL
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 16期号:6页码:623-634 |
| 关键词 | Turbot Paralichthys Olivaceus Heterologous Sperm Gynogenesis Cold Shock |
| ISSN号 | 0967-6120 |
| DOI | 10.1007/s10499-008-9173-y |
| 文献子类 | Article |
| 英文摘要 | Turbot Scophthalmus maximus exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. In this paper, gynogenetic diploids of turbot were induced by activating egg development with ultraviolet (UV)-irradiated left-eyed flounder Paralichthys olivaceus sperm combined with cold shock to prevent extrusion of the second polar body. The results of UV irradiation experiments showed that survival, motility, and duration of activity of P. olivaceus sperm generally decreased with increase in UV dose. The typical Hertwig's effect was observed after fertilized turbot eggs with UV-irradiated P. olivaceus sperm and the optimal UV dose for gynogenetic haploid production was 36,000 erg mm(-2). At 15 degrees C, appropriate timing of cold shock for retention of the second polar body in turbot eggs was at 6 min after fertilization. Results of different combinations of two shock temperatures (1 or 3 degrees C) and four shock durations (15, 25, 35 or 45 min) at 6 min after fertilization demonstrated that shock of 25 min at 1 degrees C gave the highest production of diploid gynogens (39.58% relative to its diploid control). The results of this study reveal that the use of UV-irradiated P. olivaceus sperm for activation of turbot eggs and cold shock for polar body retention is an effective method to produce gynogenetic offspring.; Turbot Scophthalmus maximus exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. In this paper, gynogenetic diploids of turbot were induced by activating egg development with ultraviolet (UV)-irradiated left-eyed flounder Paralichthys olivaceus sperm combined with cold shock to prevent extrusion of the second polar body. The results of UV irradiation experiments showed that survival, motility, and duration of activity of P. olivaceus sperm generally decreased with increase in UV dose. The typical Hertwig's effect was observed after fertilized turbot eggs with UV-irradiated P. olivaceus sperm and the optimal UV dose for gynogenetic haploid production was 36,000 erg mm(-2). At 15 degrees C, appropriate timing of cold shock for retention of the second polar body in turbot eggs was at 6 min after fertilization. Results of different combinations of two shock temperatures (1 or 3 degrees C) and four shock durations (15, 25, 35 or 45 min) at 6 min after fertilization demonstrated that shock of 25 min at 1 degrees C gave the highest production of diploid gynogens (39.58% relative to its diploid control). The results of this study reveal that the use of UV-irradiated P. olivaceus sperm for activation of turbot eggs and cold shock for polar body retention is an effective method to produce gynogenetic offspring. |
| 学科主题 | Fisheries |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000260368100012 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5699]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 266071, Peoples R China 3.Huaihai Inst Technol, Key Lab Marine Biotechnol Jiangsu Prov, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jian-He,You, Feng,Sun, Wei,et al. Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm[J]. AQUACULTURE INTERNATIONAL,2008,16(6):623-634. |
| APA | Xu, Jian-He,You, Feng,Sun, Wei,Yan, Bin-Lun,Zhang, Pei-Jun,&Jing, Bi-Xiang.(2008).Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm.AQUACULTURE INTERNATIONAL,16(6),623-634. |
| MLA | Xu, Jian-He,et al."Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm".AQUACULTURE INTERNATIONAL 16.6(2008):623-634. |
入库方式： OAI收割
来源：海洋研究所
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