Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli
文献类型：期刊论文
| 作者 | Yang, Yu1,2; Ge, Baosheng3; Guan, Xiangyu4; Zhang, Weijie1,2; Qin, Song1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 30期号:6页码:1001-1004 |
| 关键词 | Allophycocyanin Escherichia Coli Fluorescence Recombinant Protein |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-008-9644-2 |
| 文献子类 | Article |
| 英文摘要 | Allophycocyanin ( APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit ( holo- ApcA) was constructed, resulting in over- expression of a fluorescent holo- ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 gl(-1), and the expression of holo- HT- ApcA was up to 1 gl(-1) broth. The recombinant protein showed similar spectral features to native APC.; Allophycocyanin ( APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit ( holo- ApcA) was constructed, resulting in over- expression of a fluorescent holo- ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 gl(-1), and the expression of holo- HT- ApcA was up to 1 gl(-1) broth. The recombinant protein showed similar spectral features to native APC. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000255190300005 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5703]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Peoples R China 4.Ocean Univ China, Qingdao 266003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yu,Ge, Baosheng,Guan, Xiangyu,et al. Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2008,30(6):1001-1004. |
| APA | Yang, Yu,Ge, Baosheng,Guan, Xiangyu,Zhang, Weijie,&Qin, Song.(2008).Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli.BIOTECHNOLOGY LETTERS,30(6),1001-1004. |
| MLA | Yang, Yu,et al."Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli".BIOTECHNOLOGY LETTERS 30.6(2008):1001-1004. |
入库方式： OAI收割
来源：海洋研究所
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