A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates
文献类型:期刊论文
作者 | Zhang, Liusuo; Yang, Changjian; Zhang, Yang; Li, Li; Zhang, Xiaoming; Zhang, Qingli; Xiang, Jianhai |
刊名 | GENETICA |
出版日期 | 2007-09-01 |
卷号 | 131期号:1页码:37-49 |
ISSN号 | 0016-6707 |
关键词 | Linkage Map Litopenaeus Vannamei Aflp Microsatellite Sex |
DOI | 10.1007/s10709-006-9111-8 |
文献子类 | Article |
英文摘要 | Pacific white shrimp (Litopenaeus vannamei) is the leading species farmed in the Western Hemisphere and an economically important aquaculture species in China. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP) and microsatellite markers. One hundred and eight select AFLP primer combinations and 30 polymorphic microsatellite markers produced 2071 markers that were polymorphic in either of the parents and segregated in the progeny. Of these segregating markers, 319 were mapped to 45 linkage groups of the female framework map, covering a total of 4134.4 cM; and 267 markers were assigned to 45 linkage groups of the male map, covering a total of 3220.9 cM. High recombination rates were found in both parental maps. A sex-linked microsatellite marker was mapped on the female map with 6.6 cM to sex and a LOD of 17.8, two other microsatellite markers were also linked with both 8.6 cM to sex and LOD score of 14.3 and 16.4. The genetic maps presented here will serve as a basis for the construction of a high-resolution genetic map, quantitative trait loci (QTLs) detection, marker-assisted selection (MAS) and comparative genome mapping.; Pacific white shrimp (Litopenaeus vannamei) is the leading species farmed in the Western Hemisphere and an economically important aquaculture species in China. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP) and microsatellite markers. One hundred and eight select AFLP primer combinations and 30 polymorphic microsatellite markers produced 2071 markers that were polymorphic in either of the parents and segregated in the progeny. Of these segregating markers, 319 were mapped to 45 linkage groups of the female framework map, covering a total of 4134.4 cM; and 267 markers were assigned to 45 linkage groups of the male map, covering a total of 3220.9 cM. High recombination rates were found in both parental maps. A sex-linked microsatellite marker was mapped on the female map with 6.6 cM to sex and a LOD of 17.8, two other microsatellite markers were also linked with both 8.6 cM to sex and LOD score of 14.3 and 16.4. The genetic maps presented here will serve as a basis for the construction of a high-resolution genetic map, quantitative trait loci (QTLs) detection, marker-assisted selection (MAS) and comparative genome mapping. |
学科主题 | Genetics & Heredity |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000248886600005 |
公开日期 | 2010-12-24 |
源URL | [http://ir.qdio.ac.cn/handle/337002/5713] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Liusuo,Yang, Changjian,Zhang, Yang,et al. A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates[J]. GENETICA,2007,131(1):37-49. |
APA | Zhang, Liusuo.,Yang, Changjian.,Zhang, Yang.,Li, Li.,Zhang, Xiaoming.,...&Xiang, Jianhai.(2007).A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates.GENETICA,131(1),37-49. |
MLA | Zhang, Liusuo,et al."A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates".GENETICA 131.1(2007):37-49. |
入库方式: OAI收割
来源:海洋研究所
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