A novel tumor necrosis factor ligand superfamily member (CsTL) from Ciona savignyi: Molecular identification and expression analysis
文献类型：期刊论文
| 作者 | Zhang, Xiaoming1,2; Luan, Wei1,2; Jin, Songjun1; Xiang, Jianhai1
|
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 32期号:11页码:1362-1373 |
| 关键词 | Tumor Necrosis Factor (Tnf) Ligand Superfamily Molecular Clone Expression Recombinant Protein Cytotoxicity Ciona Savignyi |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2008.05.009 |
| 文献子类 | Article |
| 英文摘要 | A novel invertebrate TNF ligand was identified and characterized in Ciona savignyi. The CsTL cDNA consisted of 995 nucleotides and encoded 281 amino acids. A conserved TNF family signature and several motifs of TNF ligand superfamily were identified in deduced amino acid sequence of CsTL. Phylogenetic analysis grouped CsTL, CiTNF (predicted TNF ligand superfamily homolog in Ciona intestinalis) and urchin TL1A with their own cluster apart from mammalian TNF alpha, LTA, TNFSF15 and fish TNFa proteins. Expression studies demonstrated that CsTL mRNA is present in all tested tissues from unchallenged ascidians and its expression was significantly upregulated in hemocytes following LIPS injection. The recombinant CsTL protein expressed using a baculovirus expression system showed potential cytotoxic activity in L929 cells. Present results indicated that TNF ligand superfamity molecules are present in marine invertebrates. (C) 2008 Elsevier Ltd. All rights reserved.; A novel invertebrate TNF ligand was identified and characterized in Ciona savignyi. The CsTL cDNA consisted of 995 nucleotides and encoded 281 amino acids. A conserved TNF family signature and several motifs of TNF ligand superfamily were identified in deduced amino acid sequence of CsTL. Phylogenetic analysis grouped CsTL, CiTNF (predicted TNF ligand superfamily homolog in Ciona intestinalis) and urchin TL1A with their own cluster apart from mammalian TNF alpha, LTA, TNFSF15 and fish TNFa proteins. Expression studies demonstrated that CsTL mRNA is present in all tested tissues from unchallenged ascidians and its expression was significantly upregulated in hemocytes following LIPS injection. The recombinant CsTL protein expressed using a baculovirus expression system showed potential cytotoxic activity in L929 cells. Present results indicated that TNF ligand superfamity molecules are present in marine invertebrates. (C) 2008 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000259469700010 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5719]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoming,Luan, Wei,Jin, Songjun,et al. A novel tumor necrosis factor ligand superfamily member (CsTL) from Ciona savignyi: Molecular identification and expression analysis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2008,32(11):1362-1373. |
| APA | Zhang, Xiaoming,Luan, Wei,Jin, Songjun,&Xiang, Jianhai.(2008).A novel tumor necrosis factor ligand superfamily member (CsTL) from Ciona savignyi: Molecular identification and expression analysis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,32(11),1362-1373. |
| MLA | Zhang, Xiaoming,et al."A novel tumor necrosis factor ligand superfamily member (CsTL) from Ciona savignyi: Molecular identification and expression analysis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 32.11(2008):1362-1373. |
入库方式： OAI收割
来源：海洋研究所
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