Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Zhang, Jiquan; Li, Fuhua; Wang, Zaizhao; Xiang, Jianhai
|
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2007-01-20 |
| 卷号 | 127期号:4页码:605-614 |
| 关键词 | Fenneropenaeus Chinensis Crustin-like Gene Prokaryotic Expression Function Identification |
| ISSN号 | 0168-1656 |
| DOI | 10.1016/j.jbiotec.2006.08.013 |
| 文献子类 | Article |
| 英文摘要 | Antimicrobial peptides or proteins (AMPs) are proved to be one of the most important humoral factors to resist pathogen infection. As an antimicrobial protein, crustin had been described in invertebrates as a component of the innate immune system. A crustin-like gene (CruFc) was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5'-RACE PCR. The full-length cDNA consists of 523 with 405 bp open reading frame encoding 134 amino acids and the deduced peptide contains a putative signal peptide of 17 amino acids. The sequence also contains a whey-acidic protein (WAP) domain at the C-terminal. Transcripts of CruFc were mainly detected in haemocytes and gill by RT-PCR analysis. In addition, another full-length cDNA named CshFc was also cloned from haemocytes of Chinese shrimp and its inferred amino acid sequence lacks the WAP-type 'four-disulfide core' domain. The fusion proteins containing CruFc and CshFc were, respectively, produced and the antimicrobial assays revealed that the recombinant CruFc could inhibit the growth of grain-positive bacteria in vitro but the recombinant CshFc could not inhibit at the same conditions. The difference of antimicrobial activity between recombinant CruFc and CshFc provides the evidence that the four-disulfide core domain of crustin may play an important role in its biological function. (c) 2006 Elsevier B.V. All rights reserved.; Antimicrobial peptides or proteins (AMPs) are proved to be one of the most important humoral factors to resist pathogen infection. As an antimicrobial protein, crustin had been described in invertebrates as a component of the innate immune system. A crustin-like gene (CruFc) was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5'-RACE PCR. The full-length cDNA consists of 523 with 405 bp open reading frame encoding 134 amino acids and the deduced peptide contains a putative signal peptide of 17 amino acids. The sequence also contains a whey-acidic protein (WAP) domain at the C-terminal. Transcripts of CruFc were mainly detected in haemocytes and gill by RT-PCR analysis. In addition, another full-length cDNA named CshFc was also cloned from haemocytes of Chinese shrimp and its inferred amino acid sequence lacks the WAP-type 'four-disulfide core' domain. The fusion proteins containing CruFc and CshFc were, respectively, produced and the antimicrobial assays revealed that the recombinant CruFc could inhibit the growth of grain-positive bacteria in vitro but the recombinant CshFc could not inhibit at the same conditions. The difference of antimicrobial activity between recombinant CruFc and CshFc provides the evidence that the four-disulfide core domain of crustin may play an important role in its biological function. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000243704600005 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5725]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis[J]. JOURNAL OF BIOTECHNOLOGY,2007,127(4):605-614. |
| APA | Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,&Xiang, Jianhai.(2007).Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis.JOURNAL OF BIOTECHNOLOGY,127(4),605-614. |
| MLA | Zhang, Jiquan,et al."Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis".JOURNAL OF BIOTECHNOLOGY 127.4(2007):605-614. |
入库方式： OAI收割
来源：海洋研究所
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