Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris
文献类型：期刊论文
| 作者 | Zhang, Jiquan; Li, Fuhua; Wang, Zaizhao; Xiang, Jianhai
|
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 29期号:5页码:813-817 |
| 关键词 | Crustin-like Protein Fenneropenaeus Chinensis Pichia Pastoris |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-007-9317-6 |
| 文献子类 | Article |
| 英文摘要 | A crustin-like protein (CruFc) from Fenneropenaeus chinensis was expressed in Pichia pastoris and then purified to electrophoretic homogeneity on a Sephacryl S-100 column with a band corresponding to the expected one (13 kDa) shown by 15% SDS-PAGE. Western blot indicated that the rCruFc specifically reacted with polyclonal rabbit anti-Fenneropenaeus chinensis CruFc. Production in a 5 l bioreactor gave 237 mg rCruFc/l. Antimicrobial assay revealed that 4 mu M rCruFc inhibited growth of Staphylococcus aureus.; A crustin-like protein (CruFc) from Fenneropenaeus chinensis was expressed in Pichia pastoris and then purified to electrophoretic homogeneity on a Sephacryl S-100 column with a band corresponding to the expected one (13 kDa) shown by 15% SDS-PAGE. Western blot indicated that the rCruFc specifically reacted with polyclonal rabbit anti-Fenneropenaeus chinensis CruFc. Production in a 5 l bioreactor gave 237 mg rCruFc/l. Antimicrobial assay revealed that 4 mu M rCruFc inhibited growth of Staphylococcus aureus. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000245519800020 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5735]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris[J]. BIOTECHNOLOGY LETTERS,2007,29(5):813-817. |
| APA | Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,&Xiang, Jianhai.(2007).Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris.BIOTECHNOLOGY LETTERS,29(5),813-817. |
| MLA | Zhang, Jiquan,et al."Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris".BIOTECHNOLOGY LETTERS 29.5(2007):813-817. |
入库方式： OAI收割
来源：海洋研究所
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