Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.
文献类型：期刊论文
| 作者 | Zhang, Jiquan; Sun, Yuying |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 29期号:8页码:1221-1225 |
| 关键词 | Chitosanase Chitooligosaccharides Chitosan Cloning Microbacterium Sp. |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-007-9373-y |
| 文献子类 | Article |
| 英文摘要 | A gene encoding a chitosanase (mschito) was cloned from Microbacterium, sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase.; A gene encoding a chitosanase (mschito) was cloned from Microbacterium, sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000248224600012 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5739]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Huaihai Inst Technol, Key Lab Marine Biotechnol Jiangsu Province, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Sun, Yuying. Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.[J]. BIOTECHNOLOGY LETTERS,2007,29(8):1221-1225. |
| APA | Zhang, Jiquan,&Sun, Yuying.(2007).Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp..BIOTECHNOLOGY LETTERS,29(8),1221-1225. |
| MLA | Zhang, Jiquan,et al."Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.".BIOTECHNOLOGY LETTERS 29.8(2007):1221-1225. |
入库方式： OAI收割
来源：海洋研究所
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