A novel polypeptide from shark cartilage with potent anti-angiogenic activity
文献类型：期刊论文
| 作者 | Zheng, Lanhong; Ling, Peixue; Wang, Zheng; Niu, Rongli; Hu, Chaoxin; Zhang, Tianmin; Lin, Xiukun |
| 刊名 | CANCER BIOLOGY & THERAPY
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 6期号:5页码:775-780 |
| 关键词 | Anti-angiogenic Activity Isolation And Purification N-terminal Sequence Shark Cartilage Polypeptide Anti-cancer |
| ISSN号 | 1538-4047 |
| 文献子类 | Article |
| 英文摘要 | Using guanidine-HCl extraction, acetone precipitation, ultra-filtration and chromatography, a novel polypeptide with potent anti-angiogenic activity was purified from cartilage of the shark, Prionace glauca. N-terminal amino acid sequence analysis and SDS-PAGE revealed that the substance is a novel polypeptide with MW 15500 (PG155). The anti-angiogenic effects of PG155 were evaluated using zebrafish embryos model in vivo. Treatment of the embryos with 20 mu g/ml PG155 resulted in a significant reduction in the growth of subintestinal vessels (SIVs). A higher dose resulted in almost complete inhibition of SIV growth, as observed by endogenous alkaline phosphatase (EAP) staining assay. An in vitro transwell experiment revealed that the polypeptide inhibited vascular endothelial growth factor (VEGF) induced migration and tubulogenesis of human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs in 20 mu g/ml PG155 significantly decreased the density of migrated cells. Almost complete inhibition of cell migration was found when HUVECs were treated with 40-80 mu g/ml PG155. PG155 (20 mu g/ml) markedly inhibited the tube formation of HUVECs and a dose-dependent effect was also found when treatment of HUVECs with PG155 at the concentration from 20 to 160 mu g/ml.; Using guanidine-HCl extraction, acetone precipitation, ultra-filtration and chromatography, a novel polypeptide with potent anti-angiogenic activity was purified from cartilage of the shark, Prionace glauca. N-terminal amino acid sequence analysis and SDS-PAGE revealed that the substance is a novel polypeptide with MW 15500 (PG155). The anti-angiogenic effects of PG155 were evaluated using zebrafish embryos model in vivo. Treatment of the embryos with 20 mu g/ml PG155 resulted in a significant reduction in the growth of subintestinal vessels (SIVs). A higher dose resulted in almost complete inhibition of SIV growth, as observed by endogenous alkaline phosphatase (EAP) staining assay. An in vitro transwell experiment revealed that the polypeptide inhibited vascular endothelial growth factor (VEGF) induced migration and tubulogenesis of human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs in 20 mu g/ml PG155 significantly decreased the density of migrated cells. Almost complete inhibition of cell migration was found when HUVECs were treated with 40-80 mu g/ml PG155. PG155 (20 mu g/ml) markedly inhibited the tube formation of HUVECs and a dose-dependent effect was also found when treatment of HUVECs with PG155 at the concentration from 20 to 160 mu g/ml. |
| 学科主题 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000248259900034 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5765]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Biopharmaceut Inst Shandong Prov, Jinan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Lanhong,Ling, Peixue,Wang, Zheng,et al. A novel polypeptide from shark cartilage with potent anti-angiogenic activity[J]. CANCER BIOLOGY & THERAPY,2007,6(5):775-780. |
| APA | Zheng, Lanhong.,Ling, Peixue.,Wang, Zheng.,Niu, Rongli.,Hu, Chaoxin.,...&Lin, Xiukun.(2007).A novel polypeptide from shark cartilage with potent anti-angiogenic activity.CANCER BIOLOGY & THERAPY,6(5),775-780. |
| MLA | Zheng, Lanhong,et al."A novel polypeptide from shark cartilage with potent anti-angiogenic activity".CANCER BIOLOGY & THERAPY 6.5(2007):775-780. |
入库方式： OAI收割
来源：海洋研究所
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