The encysted dormant embryo proteome of Artemia sinica
文献类型：期刊论文
| 作者 | Zhou, Qian1,2; Wu, Changgong1; Dong, Bo1; Liu, Fengqi3; Xiang, Jianhai1
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| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 10期号:4页码:438-446 |
| 关键词 | Proteome Diapause Embryo 2d-e Mass Spectrometry Artemia Sinica |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-007-9079-0 |
| 文献子类 | Article |
| 英文摘要 | The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4-7 and a molecular weight range of 10-100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions.; The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4-7 and a molecular weight range of 10-100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000256434600011 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5769]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qian,Wu, Changgong,Dong, Bo,et al. The encysted dormant embryo proteome of Artemia sinica[J]. MARINE BIOTECHNOLOGY,2008,10(4):438-446. |
| APA | Zhou, Qian,Wu, Changgong,Dong, Bo,Liu, Fengqi,&Xiang, Jianhai.(2008).The encysted dormant embryo proteome of Artemia sinica.MARINE BIOTECHNOLOGY,10(4),438-446. |
| MLA | Zhou, Qian,et al."The encysted dormant embryo proteome of Artemia sinica".MARINE BIOTECHNOLOGY 10.4(2008):438-446. |
入库方式： OAI收割
来源：海洋研究所
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