A novel serine protease with clip domain from scallop Chlamys farreri
文献类型：期刊论文
| 作者 | Zhu, Ling1,2; Song, Linsheng1; Mao, Yuze3; Zhao, Jiangmin1; Li, Chenghua1; Xu, Wei1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 35期号:2页码:257-264 |
| 关键词 | Chlamys Farreri Clip Domain Immune Response Mrna Expression Serine Protease Tissue Distribution |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-007-9078-2 |
| 文献子类 | Article |
| 英文摘要 | The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of 1,152 bp, including a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 81 bp with a canonical polyadenylation signal sequence AATAAA and a poly(A) tail, and an open reading frame of 1,008 bp encoding a polypeptide of 336 amino acids with a putative signal peptide of 19 amino acids. The deduced amino acid sequence of Cf SP contained an amino-terminal clip domain with three disulfide bonds formed six conserved Cys residues, a carboxyl-terminal trypsin-like domain with the conserved His-Asp-Ser catalytic triad, and a low complexity linker sequence. The Cf SP was strongly expressed in hemocytes and the mRNA expression of Cf SP was up-regulated and increased 3.2-fold and 2.6-fold at 16 h after injection of Vibrio anguillarum and Micrococcus luteus. The results suggested that Cf SP gene might be involved in immune response of Gram-negative and Gram-positive microbial infection in scallop.; The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of 1,152 bp, including a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 81 bp with a canonical polyadenylation signal sequence AATAAA and a poly(A) tail, and an open reading frame of 1,008 bp encoding a polypeptide of 336 amino acids with a putative signal peptide of 19 amino acids. The deduced amino acid sequence of Cf SP contained an amino-terminal clip domain with three disulfide bonds formed six conserved Cys residues, a carboxyl-terminal trypsin-like domain with the conserved His-Asp-Ser catalytic triad, and a low complexity linker sequence. The Cf SP was strongly expressed in hemocytes and the mRNA expression of Cf SP was up-regulated and increased 3.2-fold and 2.6-fold at 16 h after injection of Vibrio anguillarum and Micrococcus luteus. The results suggested that Cf SP gene might be involved in immune response of Gram-negative and Gram-positive microbial infection in scallop. |
| 学科主题 | Biochemistry & Molecular Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000256082300024 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5771]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Key Lab Trop Marine Environm Dynam LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Mariculture Ecol Div, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Ling,Song, Linsheng,Mao, Yuze,et al. A novel serine protease with clip domain from scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):257-264. |
| APA | Zhu, Ling,Song, Linsheng,Mao, Yuze,Zhao, Jiangmin,Li, Chenghua,&Xu, Wei.(2008).A novel serine protease with clip domain from scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,35(2),257-264. |
| MLA | Zhu, Ling,et al."A novel serine protease with clip domain from scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 35.2(2008):257-264. |
入库方式： OAI收割
来源：海洋研究所
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