Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)
文献类型：期刊论文
| 作者 | Du, XJ; Wang, JX ; Liu, N; Zhao, XF; Li, FH; Xiang, JH
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| 刊名 | MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 43期号:10页码:1633-1644 |
| 关键词 | Peritrophin-like Gene Expression Pattern Fleshy Prawn Fenneropenaeus Chinensis Chitin Binding Activity Immune Defense |
| ISSN号 | 0161-5890 |
| DOI | 10.1016/j.molimm.2005.09.018 |
| 文献子类 | Article |
| 英文摘要 | Peritrophin, one of the components of the peritrophic matrix, was first isolated from the intestine of insects. It is thought to protect insects from invasion of microorganisms and to stimulate digestion of food. Peritrophin-like proteins have also been found in crustaceans, as a component of the egg layer. In this study, one fragment of the peritrophin-like gene was obtained from fleshy prawn (Chinese shrimp) (Fenneropenaeus chinensis) by panning the T7 phage display library constructed with the shrimp hemocyte cDNA. The total sequence of the peritrophin cDNA was cloned by modified SMART cDNA and LD-PCR methods. The full cDNA is 1048 bp and the deduced protein is composed of 274 amino acids, including 21 amino acid signal peptide, and four peritrophin A domains and the latter three forming three chitin-binding domains. Similarity analysis results showed that the peritrophin-like protein from F chinensis has significant similarities with peritrophin-like and cortical rod proteins from other shrimp. It was inducing expression in hemocytes, heart, stomach, gut, and gills of the infected shrimp, and constitutive expression in the ovaries. No expression signal was detected in the hepatopancreas of either infected or noninfected shrimp. The recombinant peritrophin-like protein has the activity of binding Gram-negative bacteria and strong binding activity to chitin. Therefore, the bacteria and chitin binding activities of the peritrophin-like protein suggest that it may plays a role in immune defense and other physiological resposes. (c) 2005 Elsevier Ltd. All rights reserved.; Peritrophin, one of the components of the peritrophic matrix, was first isolated from the intestine of insects. It is thought to protect insects from invasion of microorganisms and to stimulate digestion of food. Peritrophin-like proteins have also been found in crustaceans, as a component of the egg layer. In this study, one fragment of the peritrophin-like gene was obtained from fleshy prawn (Chinese shrimp) (Fenneropenaeus chinensis) by panning the T7 phage display library constructed with the shrimp hemocyte cDNA. The total sequence of the peritrophin cDNA was cloned by modified SMART cDNA and LD-PCR methods. The full cDNA is 1048 bp and the deduced protein is composed of 274 amino acids, including 21 amino acid signal peptide, and four peritrophin A domains and the latter three forming three chitin-binding domains. Similarity analysis results showed that the peritrophin-like protein from F chinensis has significant similarities with peritrophin-like and cortical rod proteins from other shrimp. It was inducing expression in hemocytes, heart, stomach, gut, and gills of the infected shrimp, and constitutive expression in the ovaries. No expression signal was detected in the hepatopancreas of either infected or noninfected shrimp. The recombinant peritrophin-like protein has the activity of binding Gram-negative bacteria and strong binding activity to chitin. Therefore, the bacteria and chitin binding activities of the peritrophin-like protein suggest that it may plays a role in immune defense and other physiological resposes. (c) 2005 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Immunology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000236450900015 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5821]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, XJ,Wang, JX,Liu, N,et al. Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)[J]. MOLECULAR IMMUNOLOGY,2006,43(10):1633-1644. |
| APA | Du, XJ,Wang, JX,Liu, N,Zhao, XF,Li, FH,&Xiang, JH.(2006).Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis).MOLECULAR IMMUNOLOGY,43(10),1633-1644. |
| MLA | Du, XJ,et al."Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)".MOLECULAR IMMUNOLOGY 43.10(2006):1633-1644. |
入库方式： OAI收割
来源：海洋研究所
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