Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor
文献类型：期刊论文
| 作者 | Gao, Jiangtao; Qin, Song; Zhang, Yicheng |
| 刊名 | CHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 122期号:1-2页码:11-14 |
| 关键词 | Gametophyte Cells Hbsag Laminaria Japonica Ph Process Conditions Stirred-tank Bioreactor |
| ISSN号 | 1385-8947 |
| DOI | 10.1016/j.cej.2006.05.004 |
| 文献子类 | Article |
| 英文摘要 | Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved.; Batch cultivation for transgenic kelp gametophyte cells was investigated in an online controlled 5 L stirred-tank photo-bioreactor to rapidly optimize the process conditions by monitoring the rate of increase of pH. The transgenic kelp gametophytes with heterologous gene encoding hepatitis B surface antigen (HBsAg) could rapidly grow in the bioreactor. Optimal temperature and agitation rate for bioreactor cultivation of gametophytes were 15 degrees C and 200 rpm. Optimal incident light intensities depended on the initial cell densities. (c) 2006 Elsevier B.V. All fights reserved. |
| 学科主题 | Engineering, Environmental ; Engineering, Chemical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000240586000002 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5831]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Jiangtao,Qin, Song,Zhang, Yicheng. Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor[J]. CHEMICAL ENGINEERING JOURNAL,2006,122(1-2):11-14. |
| APA | Gao, Jiangtao,Qin, Song,&Zhang, Yicheng.(2006).Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor.CHEMICAL ENGINEERING JOURNAL,122(1-2),11-14. |
| MLA | Gao, Jiangtao,et al."Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor".CHEMICAL ENGINEERING JOURNAL 122.1-2(2006):11-14. |
入库方式： OAI收割
来源：海洋研究所
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