Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli
文献类型：期刊论文
| 作者 | Ge, Baosheng; Qin, Song; Han, Lu; Lin, Fan; Ren, Yuhong |
| 刊名 | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 84期号:3页码:175-180 |
| 关键词 | Antioxidant Recombinant Allophycocyanin Escherichia Coli Anti-tumor Hydroxyl Radicals |
| ISSN号 | 1011-1344 |
| DOI | 10.1016/j.jphotobiol.2006.02.008 |
| 文献子类 | Article |
| 英文摘要 | Allophycocyanin (A-PC) is the main core component of phycobilisome found in blue-green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 x His-tag and their alpha or beta subunits can scavenge hydroxyl radicals successfully, and the separated g or beta subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin. (c) 2006 Elsevier B.V. All rights reserved.; Allophycocyanin (A-PC) is the main core component of phycobilisome found in blue-green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 x His-tag and their alpha or beta subunits can scavenge hydroxyl radicals successfully, and the separated g or beta subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000240212600002 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5833]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Baosheng,Qin, Song,Han, Lu,et al. Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2006,84(3):175-180. |
| APA | Ge, Baosheng,Qin, Song,Han, Lu,Lin, Fan,&Ren, Yuhong.(2006).Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,84(3),175-180. |
| MLA | Ge, Baosheng,et al."Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 84.3(2006):175-180. |
入库方式： OAI收割
来源：海洋研究所
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