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Genetic transformation in Kappaphycus alvarezii using micro-particle bombardment: a potential strategy for germplasm improvement

文献类型：期刊论文

作者Wang, Jinfeng1,2; Jiang, Peng1; Cui, Yulin1,2; Deng, Xiangyuan1,2; Li, Fuchao1; Liu, Jianguo1; Qin, Song1
刊名AQUACULTURE INTERNATIONAL
出版日期2010-10-01
卷号18期号:6页码:1027-1034
关键词Genetic Transformation Lacz Micro-particle Bombardment Sv40 Promoter Kappaphycus Alvarezii Transgenic Breeding
ISSN号0967-6120
DOI10.1007/s10499-010-9320-0
文献子类Article
英文摘要This study investigated the delivery of a SV40 promoter driving lacZ gene into cells of Kappaphycus alvarezii using particle bombardment. Thallus pieces 0.5-0.8 mm in diameter and 1 cm in length were prepared as gene recipients. Bombardment parameters of 450 psi (rupture pressures) x 6 cm (particle travel distances), 650 psi x 6 cm, 1,100 psi x 6 cm and 1,100 psi x 9 cm were used. A significant increase in transformation efficiency from about 33% under the rupture pressure of 450 psi to 87% at 650 psi was observed in transformed thalli. Most of the positive cells appeared in epidermal cells bombarded at 450 psi, whereas positive signals were seen in both epidermal and medullary cells at 650 psi. No positive transient expression was detected at a bombardment of 1,100 psi, or in negative or blank controls. For the conditions tested, the best parameter was obtained at 650 psi at a distance of 6 cm. Thus, the strategy of taking vegetative thalli as recipients, using particle bombardment, and combining this with micro-propagation, together with developing an in vivo selectable marker, is a viable way to produce stable transformants, to eliminate chimeric expression, and to achieve transgenic breeding in K. alvarezii.; This study investigated the delivery of a SV40 promoter driving lacZ gene into cells of Kappaphycus alvarezii using particle bombardment. Thallus pieces 0.5-0.8 mm in diameter and 1 cm in length were prepared as gene recipients. Bombardment parameters of 450 psi (rupture pressures) x 6 cm (particle travel distances), 650 psi x 6 cm, 1,100 psi x 6 cm and 1,100 psi x 9 cm were used. A significant increase in transformation efficiency from about 33% under the rupture pressure of 450 psi to 87% at 650 psi was observed in transformed thalli. Most of the positive cells appeared in epidermal cells bombarded at 450 psi, whereas positive signals were seen in both epidermal and medullary cells at 650 psi. No positive transient expression was detected at a bombardment of 1,100 psi, or in negative or blank controls. For the conditions tested, the best parameter was obtained at 650 psi at a distance of 6 cm. Thus, the strategy of taking vegetative thalli as recipients, using particle bombardment, and combining this with micro-propagation, together with developing an in vivo selectable marker, is a viable way to produce stable transformants, to eliminate chimeric expression, and to achieve transgenic breeding in K. alvarezii.
学科主题Fisheries
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语种英语
WOS记录号WOS:000282316700005
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5835]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Jinfeng,Jiang, Peng,Cui, Yulin,et al. Genetic transformation in Kappaphycus alvarezii using micro-particle bombardment: a potential strategy for germplasm improvement[J]. AQUACULTURE INTERNATIONAL,2010,18(6):1027-1034.
APA Wang, Jinfeng.,Jiang, Peng.,Cui, Yulin.,Deng, Xiangyuan.,Li, Fuchao.,...&Qin, Song.(2010).Genetic transformation in Kappaphycus alvarezii using micro-particle bombardment: a potential strategy for germplasm improvement.AQUACULTURE INTERNATIONAL,18(6),1027-1034.
MLA Wang, Jinfeng,et al."Genetic transformation in Kappaphycus alvarezii using micro-particle bombardment: a potential strategy for germplasm improvement".AQUACULTURE INTERNATIONAL 18.6(2010):1027-1034.

入库方式： OAI收割

来源：海洋研究所

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