中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea

文献类型:期刊论文

作者Li, Y. X.1; Gao, Z. Q.2; Li, D. M.3; Wang, G. C.4; Wang, F.1
刊名RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
出版日期2010-09-01
卷号57期号:5页码:609-614
ISSN号1021-4437
关键词Bangia Fusco-purpurea Photosystem Ii Fluorescence Emission Spectra Oxygen Evolution
DOI10.1134/S102144371005002X
文献子类Article
英文摘要The highly pure and active photosystem II (PSII) complex was isolated from Bangia fusco-purpurea (Dillw) Lyngb., an important economic red alga in China, through two steps of sucrose density gradient ultracentrifugation and characterized by the room absorption and fluorescence emission spectra, DCIP (2,6-dichloroindophenol) reduction, and oxygen evolution rates. The PSII complex from B. fusco-purpurea had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Moreover, the acquired PSII complex displayed high oxygen evolution (139 mu mol O(2)/(mg Chl h) in the presence of 2.5 mM 2,6-dimethybenzoqinone as an artificial acceptor and was active in photoreduction of DCIP (2,6-dichloroindophenol) by DPC (1,5-diphenylcarbazide) at 163 U/(mg Chl a h). SDS-PAGE also suggested that the purified PSII complex contained four intrinsic proteins (D1, D2, CP43, and CP47) and four extrinsic proteins (33-kD protein, 20-kD protein, cyt c-550, and 14-kD protein).; The highly pure and active photosystem II (PSII) complex was isolated from Bangia fusco-purpurea (Dillw) Lyngb., an important economic red alga in China, through two steps of sucrose density gradient ultracentrifugation and characterized by the room absorption and fluorescence emission spectra, DCIP (2,6-dichloroindophenol) reduction, and oxygen evolution rates. The PSII complex from B. fusco-purpurea had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Moreover, the acquired PSII complex displayed high oxygen evolution (139 mu mol O-2/(mg Chl h) in the presence of 2.5 mM 2,6-dimethybenzoqinone as an artificial acceptor and was active in photoreduction of DCIP (2,6-dichloroindophenol) by DPC (1,5-diphenylcarbazide) at 163 U/(mg Chl a h). SDS-PAGE also suggested that the purified PSII complex contained four intrinsic proteins (D1, D2, CP43, and CP47) and four extrinsic proteins (33-kD protein, 20-kD protein, cyt c-550, and 14-kD protein).
学科主题Plant Sciences
URL标识查看原文
语种英语
WOS记录号WOS:000281543600002
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5853]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋环流与波动重点实验室
作者单位1.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China
2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
3.Liaocheng Univ, Coll Agr, Liaocheng 252059, Peoples R China
4.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
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Li, Y. X.,Gao, Z. Q.,Li, D. M.,et al. Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea[J]. RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,2010,57(5):609-614.
APA Li, Y. X.,Gao, Z. Q.,Li, D. M.,Wang, G. C.,&Wang, F..(2010).Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea.RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,57(5),609-614.
MLA Li, Y. X.,et al."Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea".RUSSIAN JOURNAL OF PLANT PHYSIOLOGY 57.5(2010):609-614.

入库方式: OAI收割

来源:海洋研究所

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