Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis
文献类型：期刊论文
| 作者 | Liu, LP; Wu, CG; Cben, TY; Zhang, XJ ; Li, FH; Luo, W; Xiang, JH
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| 刊名 | JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
 |
| 出版日期 | 2006-05-16 |
| 卷号 | 332期号:2页码:121-134 |
| 关键词 | Apoptosis Cellular Response Ciona Intestinalis Egfp-E.prE.sing E. Coli Haemocytes Infection |
| ISSN号 | 0022-0981 |
| DOI | 10.1016/j.jembe.2005.11.022 |
| 文献子类 | Article |
| 英文摘要 | The effects of infection of EGFP-expressing Escherichia coli on the haemocytes of the ascidian Ciona intestinalis were investigated. The results showed that THC of the infected individuals changed significantly. Hyaline amoebocytes phagocytosed E. coli in 5 min and excreted lysosome particles that attached to the surface of the bacteria. Granular amoebocytes released lots of particles for Immoral immunity while stem-cell-like haemocytes remained intact. With the increase in THC, the stem-cell-like haemocytes showed division and proliferation. A small portion of hyaline amoebocytes was at early apoptosis stage I h after infection and typical apoptosis bodies emerged in granular amoebocytes. A few of the infected haemocytes showed DNA damage using SCGE assay. Flow cytometry analysis revealed an obvious apoptosis peak in infected haemocytes. In conclusion, apoptosis was found to be an important immune response of ascidian haemocytes response to bacterial infection. To our best knowledge, this is the first report of the occurrence of apoptosis of haemocytes in ascidians. (c) 2005 Elsevier B.V. All rights reserved.; The effects of infection of EGFP-expressing Escherichia coli on the haemocytes of the ascidian Ciona intestinalis were investigated. The results showed that THC of the infected individuals changed significantly. Hyaline amoebocytes phagocytosed E. coli in 5 min and excreted lysosome particles that attached to the surface of the bacteria. Granular amoebocytes released lots of particles for Immoral immunity while stem-cell-like haemocytes remained intact. With the increase in THC, the stem-cell-like haemocytes showed division and proliferation. A small portion of hyaline amoebocytes was at early apoptosis stage I h after infection and typical apoptosis bodies emerged in granular amoebocytes. A few of the infected haemocytes showed DNA damage using SCGE assay. Flow cytometry analysis revealed an obvious apoptosis peak in infected haemocytes. In conclusion, apoptosis was found to be an important immune response of ascidian haemocytes response to bacterial infection. To our best knowledge, this is the first report of the occurrence of apoptosis of haemocytes in ascidians. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Ecology ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000237767100001 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5869]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shanghai Fisheries Univ, Coll Aqua Life Sci & Technol, Shanghai 200090, Peoples R China 3.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, LP,Wu, CG,Cben, TY,et al. Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,2006,332(2):121-134. |
| APA | Liu, LP.,Wu, CG.,Cben, TY.,Zhang, XJ.,Li, FH.,...&Xiang, JH.(2006).Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis.JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,332(2),121-134. |
| MLA | Liu, LP,et al."Effects of infection of EGH-expressing Escherichia coli on haemocytes in Ciona intestinalis".JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 332.2(2006):121-134. |
入库方式： OAI收割
来源：海洋研究所
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