There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis
文献类型：期刊论文
| 作者 | Meng, CX; Liang, CW; Su, ZL; Qin, S; Tseng, CK |
| 刊名 | PHYCOLOGIA
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 45期号:2页码:218-224 |
| 关键词 | Haematococcus Pluvialis Astaxanthin Beta-carotene Ketolase 5 '-flanking Region Regulatory Elements Deletion Analysis |
| ISSN号 | 0031-8884 |
| 文献子类 | Article |
| 英文摘要 | The unicellular green alga Haematococcus pluvialis accumulates a commercially valuable astaxanthin, with levels reaching up to 4% dry weight under environmental stress. In recent years, much effort has been devoted to understanding the molecular mechanisms regulating astaxanthin biosynthetic pathways. Beta-carotene ketolase (bkt), with control being exhibited at the transcription level, plays an important role in astaxanthin biosynthesis by H. pluvialis. Here we demonstrate the presence of two separate 5'-flanking regions [1.5 kilobase (kb) and 2 kb] of bkt (bkt1 and bkt2) that possess regulatory elements similar to those of known stress-responsive genes in plants. Results of 5'-deletion constructs and transient beta-galactosidase expression assays demonstrate that there may be positive regulatory elements governing expression in the shorter promoter at -1060/-900 from the 1.5 kb 5' region, and in the longer promoter at -1838/-1219 and at -1046/ -734 from the 2 kb 5' region relative to each homologous ATG start codon. Furthermore, our present studies reveal that the first intron (+371/+497) downstream from the 1.5 kb 5' untranslated region of bkt1 may function as a negative regulatory element to regulate its own promoter.; The unicellular green alga Haematococcus pluvialis accumulates a commercially valuable astaxanthin, with levels reaching up to 4% dry weight under environmental stress. In recent years, much effort has been devoted to understanding the molecular mechanisms regulating astaxanthin biosynthetic pathways. Beta-carotene ketolase (bkt), with control being exhibited at the transcription level, plays an important role in astaxanthin biosynthesis by H. pluvialis. Here we demonstrate the presence of two separate 5'-flanking regions [1.5 kilobase (kb) and 2 kb] of bkt (bkt1 and bkt2) that possess regulatory elements similar to those of known stress-responsive genes in plants. Results of 5'-deletion constructs and transient beta-galactosidase expression assays demonstrate that there may be positive regulatory elements governing expression in the shorter promoter at -1060/-900 from the 1.5 kb 5' region, and in the longer promoter at -1838/-1219 and at -1046/ -734 from the 2 kb 5' region relative to each homologous ATG start codon. Furthermore, our present studies reveal that the first intron (+371/+497) downstream from the 1.5 kb 5' untranslated region of bkt1 may function as a negative regulatory element to regulate its own promoter. |
| 学科主题 | Plant Sciences ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235940800012 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5877]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Zhejiang Univ, Coll Life Sci, Dept Biotechnol, Hangzhou 310027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, CX,Liang, CW,Su, ZL,et al. There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis[J]. PHYCOLOGIA,2006,45(2):218-224. |
| APA | Meng, CX,Liang, CW,Su, ZL,Qin, S,&Tseng, CK.(2006).There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis.PHYCOLOGIA,45(2),218-224. |
| MLA | Meng, CX,et al."There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis".PHYCOLOGIA 45.2(2006):218-224. |
入库方式： OAI收割
来源：海洋研究所
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