Photosynthetic performance of regenerated protoplasts from disintegrated cells of Bryopsis hypnoides (Chlorophyta)
文献类型：期刊论文
| 作者 | Lue, F.1,2; Wang, G. C.1; Niu, J. F.1 |
| 刊名 | PHOTOSYNTHETICA
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 48期号:2页码:294-301 |
| 关键词 | Bryopsis Hypnoides Chlorophyll Fluorescence Imaging-pam Oxygen Measurements Protoplasts Photosynthesis |
| ISSN号 | 0300-3604 |
| DOI | 10.1007/s11099-010-0036-1 |
| 文献子类 | Article |
| 英文摘要 | The photosynthetic performances of regenerated protoplasts of Bryopsis hypnoides, which were incubated in seawater for 1, 6, 12, and 24 h, were studied using chlorophyll (Chl) fluorescence and oxygen measurements. Results showed that for the regenerated protoplasts, the pigment content, the ratios of photosynthetic rate to respiration rate, the maximal photosystem II (PSII) quantum yield (F(v)/F(m)), and the effective PSII quantum yield (I broken vertical bar(PSII)) decreased gradually along with the regeneration progress, indicated that during 24 h of regeneration there was a remarkable reduction in PSII activity of those newly formed protoplasts. We assumed that during the cultivation progress the regenerated protoplasts had different photosynthetic vigor, with only some of them able to germinate and develop into mature thalli. The above results only reflected the photosynthetic features of the regenerated protoplasts at each time point as a whole, rather than the actual photosynthetic activity of individual aggregations. Further investigation suggested a relationship between the size of regenerated protoplasts and their viability. The results showed that the middle-sized group (diameter 20-60 mu m) retained the largest number of protoplasts for 24 h of growth. The changes in F(v)/F(m) and I broken vertical bar(PSII) of the four groups of differently sized protoplasts (i.e. < 20, 20-60, 60-100, and > 100 mu m) revealed that the protoplasts 20-60 mu m in diameter had the highest potential activity of the photosynthetic light energy absorption and conversion for several hours.; The photosynthetic performances of regenerated protoplasts of Bryopsis hypnoides, which were incubated in seawater for 1, 6, 12, and 24 h, were studied using chlorophyll (Chl) fluorescence and oxygen measurements. Results showed that for the regenerated protoplasts, the pigment content, the ratios of photosynthetic rate to respiration rate, the maximal photosystem II (PSII) quantum yield (F-v/F-m), and the effective PSII quantum yield (I broken vertical bar(PSII)) decreased gradually along with the regeneration progress, indicated that during 24 h of regeneration there was a remarkable reduction in PSII activity of those newly formed protoplasts. We assumed that during the cultivation progress the regenerated protoplasts had different photosynthetic vigor, with only some of them able to germinate and develop into mature thalli. The above results only reflected the photosynthetic features of the regenerated protoplasts at each time point as a whole, rather than the actual photosynthetic activity of individual aggregations. Further investigation suggested a relationship between the size of regenerated protoplasts and their viability. The results showed that the middle-sized group (diameter 20-60 mu m) retained the largest number of protoplasts for 24 h of growth. The changes in F-v/F-m and I broken vertical bar(PSII) of the four groups of differently sized protoplasts (i.e. < 20, 20-60, 60-100, and > 100 mu m) revealed that the protoplasts 20-60 mu m in diameter had the highest potential activity of the photosynthetic light energy absorption and conversion for several hours. |
| 学科主题 | Plant Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000280947000015 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5903]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lue, F.,Wang, G. C.,Niu, J. F.. Photosynthetic performance of regenerated protoplasts from disintegrated cells of Bryopsis hypnoides (Chlorophyta)[J]. PHOTOSYNTHETICA,2010,48(2):294-301. |
| APA | Lue, F.,Wang, G. C.,&Niu, J. F..(2010).Photosynthetic performance of regenerated protoplasts from disintegrated cells of Bryopsis hypnoides (Chlorophyta).PHOTOSYNTHETICA,48(2),294-301. |
| MLA | Lue, F.,et al."Photosynthetic performance of regenerated protoplasts from disintegrated cells of Bryopsis hypnoides (Chlorophyta)".PHOTOSYNTHETICA 48.2(2010):294-301. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。