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Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing

文献类型：期刊论文

作者Xiao, Ping-ping1,2; Hu, Yong-hua1; Sun, Li1
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2010-12-01
卷号34期号:12页码:1237-1241
关键词Cystatin b Scophthalmus Maximus Macrophage Bacterial Infection
ISSN号0145-305X
DOI10.1016/j.dci.2010.07.008
文献子类Article
英文摘要Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300 bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen. blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia colt exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300 bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen. blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia colt exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题Immunology ; Zoology
URL标识查看原文
语种英语
WOS记录号WOS:000283270700001
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5915]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Xiao, Ping-ping,Hu, Yong-hua,Sun, Li. Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(12):1237-1241.
APA Xiao, Ping-ping,Hu, Yong-hua,&Sun, Li.(2010).Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(12),1237-1241.
MLA Xiao, Ping-ping,et al."Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.12(2010):1237-1241.

入库方式： OAI收割

来源：海洋研究所

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