Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians
文献类型：期刊论文
| 作者 | Song, LS; Xu, W; Li, CH; Li, HL ; Wu, LT; Xiang, JH; Guo, XM
|
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 8期号:2页码:161-169 |
| 关键词 | Argopecten Irradians Bay Scallop Cdna Library Diseases Expressed Sequence Tags Host Defense |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-005-0126-4 |
| 文献子类 | Article |
| 英文摘要 | The bay scallop, Argopecten irradians irradians, introduced from North America, has become one of the most important aquaculture species in China. Inan effort to identify scallop genes involved in host defense, a high-quality cDNA library was constructed from whole body tissues of the bay scallop. A total of 5828 successful sequencing reactions yielded 4995 expressed sequence tags (ESTs) longer than 100 bp. Cluster and assembly analyses of the ESTs identified 637 contigs (consisting of 2853 sequences) and 2142 singletons, totaling 2779 unique sequences. Basic Local Alignment Search Tool (BLAST) analysis showed that the majority (73%) of the unique sequences had no significant homology (E-value >= 0.005) to sequences in GenBank. Among the 748 sequences with significant GenBank matches, 160 (21.4%) were for genes related to metabolism, 131 (17.5%) for cell/organism defense, 124 (16.6%) for gene/protein expression, 83 (11.1%) for cell structure/motility, 70 (9.4%) for cell signaling/communication, 17 (2.3%) for cell division, and 163 (21.8%) matched to genes of unknown functions. The list of host-defense genes included many genes with known and important roles in innate defense such as lectins, defensins, proteases, protease inhibitors, heat shock proteins, antioxidants, and Toll-like receptors. The study provides a significant number of ESTs for gene discovery and candidate genes for studying host defense in scallops and other molluscs.; The bay scallop, Argopecten irradians irradians, introduced from North America, has become one of the most important aquaculture species in China. Inan effort to identify scallop genes involved in host defense, a high-quality cDNA library was constructed from whole body tissues of the bay scallop. A total of 5828 successful sequencing reactions yielded 4995 expressed sequence tags (ESTs) longer than 100 bp. Cluster and assembly analyses of the ESTs identified 637 contigs (consisting of 2853 sequences) and 2142 singletons, totaling 2779 unique sequences. Basic Local Alignment Search Tool (BLAST) analysis showed that the majority (73%) of the unique sequences had no significant homology (E-value >= 0.005) to sequences in GenBank. Among the 748 sequences with significant GenBank matches, 160 (21.4%) were for genes related to metabolism, 131 (17.5%) for cell/organism defense, 124 (16.6%) for gene/protein expression, 83 (11.1%) for cell structure/motility, 70 (9.4%) for cell signaling/communication, 17 (2.3%) for cell division, and 163 (21.8%) matched to genes of unknown functions. The list of host-defense genes included many genes with known and important roles in innate defense such as lectins, defensins, proteases, protease inhibitors, heat shock proteins, antioxidants, and Toll-like receptors. The study provides a significant number of ESTs for gene discovery and candidate genes for studying host defense in scallops and other molluscs. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000236968600007 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5919]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, LS,Xu, W,Li, CH,et al. Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians[J]. MARINE BIOTECHNOLOGY,2006,8(2):161-169. |
| APA | Song, LS.,Xu, W.,Li, CH.,Li, HL.,Wu, LT.,...&Guo, XM.(2006).Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians.MARINE BIOTECHNOLOGY,8(2),161-169. |
| MLA | Song, LS,et al."Development of expressed sequence tags from the bay scallop, Argopecten irradians irradians".MARINE BIOTECHNOLOGY 8.2(2006):161-169. |
入库方式： OAI收割
来源：海洋研究所
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