Construction and analysis of an experimental Streptococcus iniae DNA vaccine
文献类型：期刊论文
| 作者 | Sun, Yun1,2; Hu, Yong-hua1; Liu, Chun-sheng1,2; Sun, Li1 |
| 刊名 | VACCINE
 |
| 出版日期 | 2010-05-21 |
| 卷号 | 28期号:23页码:3905-3912 |
| 关键词 | Streptococcus Iniae Dna Vaccine Immune Genes Passive Immunization |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2010.03.071 |
| 文献子类 | Article |
| 英文摘要 | Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examined as a DNA vaccine in the form of plasmid pSia10, which expresses sia10 under the cytomegalovirus immediate-early promoter. In fish vaccinated with pSia10, transcription of sia10 was detected in muscle, liver, spleen, and kidney at 7, 14, 21, 28, 35, 42, and 49 days post-vaccination. In addition, production of Sia10 protein was also detected in the muscle tissues of pSia10-vaccinated fish. Fish vaccinated with pSia10 exhibited a relative percent survival (RPS) of 73.9% and 92.3%, respectively, when challenged with high and low doses (producing a cumulative mortality of 92% and 52%, respectively, in the control groups) of S. iniae. Immunological and transcriptional analyses showed that vaccination with pSia10(i) induced much stronger chemiluminescence response and significantly higher levels of nitric oxide production and acid phosphatase activity in head kidney macrophages; (ii) caused the production of specific serum antibodies, which afforded apparent immunoprotection when transferred passively into naive fish; and (iii) upregulated the expression of the genes encoding proteins that are possibly involved in both innate and adaptive immune responses. Taken together, these results indicated that pSia10 is an effective vaccine candidate and may be used in the control of S. iniae infection in aquaculture. (C) 2010 Elsevier Ltd. All rights reserved.; Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examined as a DNA vaccine in the form of plasmid pSia10, which expresses sia10 under the cytomegalovirus immediate-early promoter. In fish vaccinated with pSia10, transcription of sia10 was detected in muscle, liver, spleen, and kidney at 7, 14, 21, 28, 35, 42, and 49 days post-vaccination. In addition, production of Sia10 protein was also detected in the muscle tissues of pSia10-vaccinated fish. Fish vaccinated with pSia10 exhibited a relative percent survival (RPS) of 73.9% and 92.3%, respectively, when challenged with high and low doses (producing a cumulative mortality of 92% and 52%, respectively, in the control groups) of S. iniae. Immunological and transcriptional analyses showed that vaccination with pSia10(i) induced much stronger chemiluminescence response and significantly higher levels of nitric oxide production and acid phosphatase activity in head kidney macrophages; (ii) caused the production of specific serum antibodies, which afforded apparent immunoprotection when transferred passively into naive fish; and (iii) upregulated the expression of the genes encoding proteins that are possibly involved in both innate and adaptive immune responses. Taken together, these results indicated that pSia10 is an effective vaccine candidate and may be used in the control of S. iniae infection in aquaculture. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Medicine, Research & Experimental |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000278985600006 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5925]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yun,Hu, Yong-hua,Liu, Chun-sheng,et al. Construction and analysis of an experimental Streptococcus iniae DNA vaccine[J]. VACCINE,2010,28(23):3905-3912. |
| APA | Sun, Yun,Hu, Yong-hua,Liu, Chun-sheng,&Sun, Li.(2010).Construction and analysis of an experimental Streptococcus iniae DNA vaccine.VACCINE,28(23),3905-3912. |
| MLA | Sun, Yun,et al."Construction and analysis of an experimental Streptococcus iniae DNA vaccine".VACCINE 28.23(2010):3905-3912. |
入库方式： OAI收割
来源：海洋研究所
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