Purification and characterization of two types of chitosanase from a Microbacterium sp.
文献类型：期刊论文
| 作者 | Sun, Yuying; Liu, Wanshun; Han, Baoqin; Zhang, Jiquan; Liu, Bing |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 28期号:17页码:1393-1399 |
| 关键词 | Chitooligosaccharides Chitosan Chitosanase Endo-type Exo-type Microbacterium Sp. |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-006-9101-z |
| 文献子类 | Article |
| 英文摘要 | Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products.; Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products. |
| 学科主题 | Biotechnology & Applied Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000239813900011 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5927]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuying,Liu, Wanshun,Han, Baoqin,et al. Purification and characterization of two types of chitosanase from a Microbacterium sp.[J]. BIOTECHNOLOGY LETTERS,2006,28(17):1393-1399. |
| APA | Sun, Yuying,Liu, Wanshun,Han, Baoqin,Zhang, Jiquan,&Liu, Bing.(2006).Purification and characterization of two types of chitosanase from a Microbacterium sp..BIOTECHNOLOGY LETTERS,28(17),1393-1399. |
| MLA | Sun, Yuying,et al."Purification and characterization of two types of chitosanase from a Microbacterium sp.".BIOTECHNOLOGY LETTERS 28.17(2006):1393-1399. |
入库方式： OAI收割
来源：海洋研究所
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