中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method

文献类型:期刊论文

作者Wang, Xuliang; Wang, Di; Li, Dapeng; Duan, Delin
刊名AQUACULTURE
出版日期2006-08-31
卷号258期号:1-4页码:250-256
关键词Undaria Gametophytes Inter-simple Sequence Repeat Genetic Analysis Mantel Test Dendrogram
ISSN号0044-8486
DOI10.1016/j.aquaculture.2006.04.029
文献子类Article
英文摘要Inter-simple sequence repeat (ISSR) analysis was used to assess eleven pairs of Undaria pinnatifida (Harv.) Suringar male and female gametophytes. After screening fifty primers, 18 ISSR primers were selected for final analysis. A total of 104 loci were obtained, of which 77 were polymorphic, among the gametophytes studied. Genetic relationships were analyzed with simple matching (S), Jaccard's (J) and Dice's (D) distance coefficients. Little genetic variations were found among the selected Undaria gametophytes, for instance, the genetic distances ranging from 0.010 to 0.125 with Dice coefficients. UPGMA dendrograms showed that 11 pairs of Undaria gametophytes were distributed into five groups. Most Undaria strains cultivated in China exhibited closely genetic relationships with the strains from Japan. However, gametophytes from Qingdao appeared as distinct clades from other Undaria strains with all three distance coefficients used. Mantel test showed that the three distance measurements generated congruent clustering patterns on the same data. Our results demonstrated the feasibility of applying ISSR markers for genetic analysis of Undaria gametophytes. (c) 2006 Elsevier B.V. All rights reserved.; Inter-simple sequence repeat (ISSR) analysis was used to assess eleven pairs of Undaria pinnatifida (Harv.) Suringar male and female gametophytes. After screening fifty primers, 18 ISSR primers were selected for final analysis. A total of 104 loci were obtained, of which 77 were polymorphic, among the gametophytes studied. Genetic relationships were analyzed with simple matching (S), Jaccard's (J) and Dice's (D) distance coefficients. Little genetic variations were found among the selected Undaria gametophytes, for instance, the genetic distances ranging from 0.010 to 0.125 with Dice coefficients. UPGMA dendrograms showed that 11 pairs of Undaria gametophytes were distributed into five groups. Most Undaria strains cultivated in China exhibited closely genetic relationships with the strains from Japan. However, gametophytes from Qingdao appeared as distinct clades from other Undaria strains with all three distance coefficients used. Mantel test showed that the three distance measurements generated congruent clustering patterns on the same data. Our results demonstrated the feasibility of applying ISSR markers for genetic analysis of Undaria gametophytes. (c) 2006 Elsevier B.V. All rights reserved.
学科主题Fisheries ; Marine & Freshwater Biology
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语种英语
WOS记录号WOS:000240252900026
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5957]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xuliang,Wang, Di,Li, Dapeng,et al. Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method[J]. AQUACULTURE,2006,258(1-4):250-256.
APA Wang, Xuliang,Wang, Di,Li, Dapeng,&Duan, Delin.(2006).Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method.AQUACULTURE,258(1-4),250-256.
MLA Wang, Xuliang,et al."Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method".AQUACULTURE 258.1-4(2006):250-256.

入库方式: OAI收割

来源:海洋研究所

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