Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method
文献类型:期刊论文
作者 | Wang, Xuliang; Wang, Di; Li, Dapeng; Duan, Delin |
刊名 | AQUACULTURE
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出版日期 | 2006-08-31 |
卷号 | 258期号:1-4页码:250-256 |
关键词 | Undaria Gametophytes Inter-simple Sequence Repeat Genetic Analysis Mantel Test Dendrogram |
ISSN号 | 0044-8486 |
DOI | 10.1016/j.aquaculture.2006.04.029 |
文献子类 | Article |
英文摘要 | Inter-simple sequence repeat (ISSR) analysis was used to assess eleven pairs of Undaria pinnatifida (Harv.) Suringar male and female gametophytes. After screening fifty primers, 18 ISSR primers were selected for final analysis. A total of 104 loci were obtained, of which 77 were polymorphic, among the gametophytes studied. Genetic relationships were analyzed with simple matching (S), Jaccard's (J) and Dice's (D) distance coefficients. Little genetic variations were found among the selected Undaria gametophytes, for instance, the genetic distances ranging from 0.010 to 0.125 with Dice coefficients. UPGMA dendrograms showed that 11 pairs of Undaria gametophytes were distributed into five groups. Most Undaria strains cultivated in China exhibited closely genetic relationships with the strains from Japan. However, gametophytes from Qingdao appeared as distinct clades from other Undaria strains with all three distance coefficients used. Mantel test showed that the three distance measurements generated congruent clustering patterns on the same data. Our results demonstrated the feasibility of applying ISSR markers for genetic analysis of Undaria gametophytes. (c) 2006 Elsevier B.V. All rights reserved.; Inter-simple sequence repeat (ISSR) analysis was used to assess eleven pairs of Undaria pinnatifida (Harv.) Suringar male and female gametophytes. After screening fifty primers, 18 ISSR primers were selected for final analysis. A total of 104 loci were obtained, of which 77 were polymorphic, among the gametophytes studied. Genetic relationships were analyzed with simple matching (S), Jaccard's (J) and Dice's (D) distance coefficients. Little genetic variations were found among the selected Undaria gametophytes, for instance, the genetic distances ranging from 0.010 to 0.125 with Dice coefficients. UPGMA dendrograms showed that 11 pairs of Undaria gametophytes were distributed into five groups. Most Undaria strains cultivated in China exhibited closely genetic relationships with the strains from Japan. However, gametophytes from Qingdao appeared as distinct clades from other Undaria strains with all three distance coefficients used. Mantel test showed that the three distance measurements generated congruent clustering patterns on the same data. Our results demonstrated the feasibility of applying ISSR markers for genetic analysis of Undaria gametophytes. (c) 2006 Elsevier B.V. All rights reserved. |
学科主题 | Fisheries ; Marine & Freshwater Biology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000240252900026 |
公开日期 | 2010-12-24 |
源URL | [http://ir.qdio.ac.cn/handle/337002/5957] ![]() |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xuliang,Wang, Di,Li, Dapeng,et al. Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method[J]. AQUACULTURE,2006,258(1-4):250-256. |
APA | Wang, Xuliang,Wang, Di,Li, Dapeng,&Duan, Delin.(2006).Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method.AQUACULTURE,258(1-4),250-256. |
MLA | Wang, Xuliang,et al."Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method".AQUACULTURE 258.1-4(2006):250-256. |
入库方式: OAI收割
来源:海洋研究所
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