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Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos

文献类型：期刊论文

作者Mao, Z. Z.1; Zhang, L. L.1,2; Xu, X. Z.; Liu, Q. H.1,2; Li, J.1; Ma, D. Y.1; Xu, S. H.1; Xue, Y. P.; Xue, Q. Z.3
刊名THERIOGENOLOGY
出版日期2008-10-15
卷号70期号:7页码:1086-1092
关键词Red Seabream Embryo Cryoprotectant Toxicity Permeable And non-Permeable
ISSN号0093-691X
DOI10.1016/j.theriogenology.2008.06.028
文献子类Article
英文摘要The objectives were to investigate the effect of cryoprotectants on the hatching rate of red seabream embryos. Heart-beat embryos were immersed in: five permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), 1,2-propylene glycol (PG), and ethylene glycol (EG). in concentrations of 5-30% for 10, 30, or 60 min; and two non-permeable cryoprotectants: polyvinylpyrrolidone (PVP), and sucrose (in concentrations of 5-20% for 10 or 30 min). The embryos were then washed and incubated in filtered seawater until hatching occurred. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. In addition, PG was the least toxic permeable cryoprotectant, followed by DMSO and EG, whereas Gly and MeOH were the most toxic. At a concentration of 15% and 30 min exposure, the hatching rate of the embryos immersed in PG was 93.3 +/- 7.0% (mean +/- S.D.), however. in DMSO. EG, Gly. and MeOH, it was 82.7 +/- 10.4, 22.0 +/- 5.7, 0.0 +/- 0.0, and 0.0 +/- 0.0%, respectively. Hatching rate of embryos treated with PVP decreased (P < 0.05) with the increase of concentration and exposure time, whereas for embryos treated with sucrose, there was no significant decrease in comparison with the control at the concentrations used. (C) 2008 Elsevier Inc. All rights reserved.; The objectives were to investigate the effect of cryoprotectants on the hatching rate of red seabream embryos. Heart-beat embryos were immersed in: five permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), 1,2-propylene glycol (PG), and ethylene glycol (EG). in concentrations of 5-30% for 10, 30, or 60 min; and two non-permeable cryoprotectants: polyvinylpyrrolidone (PVP), and sucrose (in concentrations of 5-20% for 10 or 30 min). The embryos were then washed and incubated in filtered seawater until hatching occurred. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. In addition, PG was the least toxic permeable cryoprotectant, followed by DMSO and EG, whereas Gly and MeOH were the most toxic. At a concentration of 15% and 30 min exposure, the hatching rate of the embryos immersed in PG was 93.3 +/- 7.0% (mean +/- S.D.), however. in DMSO. EG, Gly. and MeOH, it was 82.7 +/- 10.4, 22.0 +/- 5.7, 0.0 +/- 0.0, and 0.0 +/- 0.0%, respectively. Hatching rate of embryos treated with PVP decreased (P < 0.05) with the increase of concentration and exposure time, whereas for embryos treated with sucrose, there was no significant decrease in comparison with the control at the concentrations used. (C) 2008 Elsevier Inc. All rights reserved.
学科主题Reproductive Biology ; Veterinary Sciences
URL标识查看原文
语种英语
WOS记录号WOS:000259750000009
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/6075]  
专题海洋研究所_海洋生物技术研发中心
海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Mao, Z. Z.,Zhang, L. L.,Xu, X. Z.,et al. Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos[J]. THERIOGENOLOGY,2008,70(7):1086-1092.
APA Mao, Z. Z..,Zhang, L. L..,Xu, X. Z..,Liu, Q. H..,Li, J..,...&Xue, Q. Z..(2008).Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos.THERIOGENOLOGY,70(7),1086-1092.
MLA Mao, Z. Z.,et al."Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos".THERIOGENOLOGY 70.7(2008):1086-1092.

入库方式： OAI收割

来源：海洋研究所

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