Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye
文献类型：期刊论文
| 作者 | Feng, Jinhua1,2; Yu, Huahua1 ; Xing, Ronge1; Liu, Song1; Wang, Lin1,2; Cai, Shengbao1,2; Li, Pengcheng1
|
| 刊名 | TOXICOLOGY IN VITRO
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 24期号:6页码:1750-1756 |
| ISSN号 | 0887-2333 |
| 关键词 | Characterization Cyanea Nozakii Kishinouye Hemolytic Activity Jellyfish Nematocyst Pla(2) Activity Stability Venom |
| DOI | 10.1016/j.tiv.2010.02.010 |
| 文献子类 | Article |
| 英文摘要 | Using a recently developed technique to extract jellyfish venom from nematocysts, the present study investigated the hemolytic activity of Cyanea nozakii Kishinouye nematocyst venom on chicken erythrocytes. Venom extract caused a significant concentration-dependent hemolytic effect. The extract could retain its activity at -80 degrees C but was unstable when kept at 4 degrees C and -20 degrees C for 2 days. The hemolytic activity was inhibited by heating within the range of 37-100 degrees C. The extract was active over a pH range of 5.0-8.63 and the pH optima for the extract was 7.8. Incubation of the venom with sphingomyelin specially inhibited hemolytic activity by up to 70%. Cu(2+) and Mn(2+) greatly reduced the hemolytic activity while Mg(2+), Sr(2+) and Ba(2+) produced a relatively low inhibiting effect on the hemolytic activity. Treatment with Ca(2+) induced a concentration-dependent increase in the hemolytic activity. In the presence of 5 mM EDTA, all the hemolytic activity was lost, however, the venom containing 1.5 mM EDTA was stable in the long-term storage. PLA(2) activity was also found in the nematocyst venom of C. nozakii. These characteristics provide us a fundamental knowledge in the C. nozakii nematocyst venom which would benefit future research. (C) 2010 Published by Elsevier Ltd.; Using a recently developed technique to extract jellyfish venom from nematocysts, the present study investigated the hemolytic activity of Cyanea nozakii Kishinouye nematocyst venom on chicken erythrocytes. Venom extract caused a significant concentration-dependent hemolytic effect. The extract could retain its activity at -80 degrees C but was unstable when kept at 4 degrees C and -20 degrees C for 2 days. The hemolytic activity was inhibited by heating within the range of 37-100 degrees C. The extract was active over a pH range of 5.0-8.63 and the pH optima for the extract was 7.8. Incubation of the venom with sphingomyelin specially inhibited hemolytic activity by up to 70%. Cu2+ and Mn2+ greatly reduced the hemolytic activity while Mg2+, Sr2+ and Ba2+ produced a relatively low inhibiting effect on the hemolytic activity. Treatment with Ca2+ induced a concentration-dependent increase in the hemolytic activity. In the presence of 5 mM EDTA, all the hemolytic activity was lost, however, the venom containing 1.5 mM EDTA was stable in the long-term storage. PLA(2) activity was also found in the nematocyst venom of C. nozakii. These characteristics provide us a fundamental knowledge in the C. nozakii nematocyst venom which would benefit future research. (C) 2010 Published by Elsevier Ltd. |
| 学科主题 | Toxicology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000282114400036 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6153]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Jinhua,Yu, Huahua,Xing, Ronge,et al. Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye[J]. TOXICOLOGY IN VITRO,2010,24(6):1750-1756. |
| APA | Feng, Jinhua.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Wang, Lin.,...&Li, Pengcheng.(2010).Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye.TOXICOLOGY IN VITRO,24(6),1750-1756. |
| MLA | Feng, Jinhua,et al."Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye".TOXICOLOGY IN VITRO 24.6(2010):1750-1756. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。