Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts
文献类型：期刊论文
| 作者 | Li, Demao1,3,4; Lue, Fang1,4; Wang, Guangce1,2; Zhou, Baicheng1 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 50期号:6页码:752-760 |
| 关键词 | Codium Fragile Protoplasm Protoplast Cell Wall |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/j.1744-7909.2008.00671.x |
| 文献子类 | Article |
| 英文摘要 | The cell organelles of the coenocytic alga Codium fragile (Sur.) Hariot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts.; The cell organelles of the coenocytic alga Codium fragile (Sur.) Hariot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000256532500014 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6287]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tainjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Liaocheng Univ, Coll Agr, Dept Food Sci, Liaocheng 252059, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Demao,Lue, Fang,Wang, Guangce,et al. Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2008,50(6):752-760. |
| APA | Li, Demao,Lue, Fang,Wang, Guangce,&Zhou, Baicheng.(2008).Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,50(6),752-760. |
| MLA | Li, Demao,et al."Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 50.6(2008):752-760. |
入库方式： OAI收割
来源：海洋研究所
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