Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Liu Fengsong1,2; Li Fuhua1; Xiang Jianhai1 ; Dong Bo1; Liu Yichen1; Zhang Xiaojun1; Zhang Liusuo1
|
| 刊名 | ACTA OCEANOLOGICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 27期号:2页码:81-92 |
| 关键词 | Crustin-like Gene Fenaeropenaeius Chinensis Molecular Cloning Expression Analysis Microbe Challenge |
| ISSN号 | 0253-505X |
| 文献子类 | Article |
| 英文摘要 | A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fenneropenaeus chinensis by 3' and 5' RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12.3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeas) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary electrophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.; A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fenneropenaeus chinensis by 3' and 5' RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12.3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeas) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary electrophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen. |
| 语种 | 英语 |
| WOS记录号 | WOS:000256131900007 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6293]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Fengsong,Li Fuhua,Xiang Jianhai,et al. Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis[J]. ACTA OCEANOLOGICA SINICA,2008,27(2):81-92. |
| APA | Liu Fengsong.,Li Fuhua.,Xiang Jianhai.,Dong Bo.,Liu Yichen.,...&Zhang Liusuo.(2008).Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis.ACTA OCEANOLOGICA SINICA,27(2),81-92. |
| MLA | Liu Fengsong,et al."Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis".ACTA OCEANOLOGICA SINICA 27.2(2008):81-92. |
入库方式： OAI收割
来源：海洋研究所
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