PRELIMINARY STUDIES ON CRYOPRESERVATION OF SYDNEY ROCK OYSTER (SACCOSTREA GLOMERATA) LARVAE
文献类型：期刊论文
| 作者 | Liu, Baozhong2 ; Li, Xiaoxu1
|
| 刊名 | JOURNAL OF SHELLFISH RESEARCH
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 27期号:5页码:1125-1128 |
| 关键词 | Cryopreservation Saccostrea Glomerata Larvae Dmso Pg Oyster |
| ISSN号 | 0730-8000 |
| 文献子类 | Article |
| 英文摘要 | In this study several parameters critical to the success of cryopreserving Sydney rock oyster (Saccostrea glomerata) larvae were investigated. They were: (1) cryoprotectants (10% dimethyl sulfoxide and 10% propylene glycol). (2) freezing protocols (with or without the seeding step). (3) larval concentrations (1,000, 3,000, 5,000, 10,000, 30,000 individuals mL(-1)). and (4) larval ages (6, 12, 24, 48 and 96 h old). The survival rates were determined as percentages of postthaw larvae performing active movements for the 6 and 12 h larvae or active cilia movement for the 24, 48 and 96 h larvae. Analyses showed that the difference in survival rates between different age classses was significant in all the experiments conducted, with the maximum survival rate being achieved in the 24-h-old larvae the postthaw survival rates of larvae cryopreserved with 10% dimethyl sulfoxide (93.1 +/- 0.2%) were significantly higher (P < 0.001) that those with 10% propylene glycol (81.5 +/- 0.4%). Differences in postthaw survival rates between different concentrations (1,000 30,000 individuals mL(-1)) were not significant within each of the three larval age classes (6-, 12-, and 24-h-old ) used.; In this study several parameters critical to the success of cryopreserving Sydney rock oyster (Saccostrea glomerata) larvae were investigated. They were: (1) cryoprotectants (10% dimethyl sulfoxide and 10% propylene glycol). (2) freezing protocols (with or without the seeding step). (3) larval concentrations (1,000, 3,000, 5,000, 10,000, 30,000 individuals mL(-1)). and (4) larval ages (6, 12, 24, 48 and 96 h old). The survival rates were determined as percentages of postthaw larvae performing active movements for the 6 and 12 h larvae or active cilia movement for the 24, 48 and 96 h larvae. Analyses showed that the difference in survival rates between different age classses was significant in all the experiments conducted, with the maximum survival rate being achieved in the 24-h-old larvae the postthaw survival rates of larvae cryopreserved with 10% dimethyl sulfoxide (93.1 +/- 0.2%) were significantly higher (P < 0.001) that those with 10% propylene glycol (81.5 +/- 0.4%). Differences in postthaw survival rates between different concentrations (1,000 30,000 individuals mL(-1)) were not significant within each of the three larval age classes (6-, 12-, and 24-h-old ) used. |
| 语种 | 英语 |
| WOS记录号 | WOS:000261712200008 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6295]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.S Australian Res & Dev Inst, W Beach, SA 5024, Australia 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Baozhong,Li, Xiaoxu. PRELIMINARY STUDIES ON CRYOPRESERVATION OF SYDNEY ROCK OYSTER (SACCOSTREA GLOMERATA) LARVAE[J]. JOURNAL OF SHELLFISH RESEARCH,2008,27(5):1125-1128. |
| APA | Liu, Baozhong,&Li, Xiaoxu.(2008).PRELIMINARY STUDIES ON CRYOPRESERVATION OF SYDNEY ROCK OYSTER (SACCOSTREA GLOMERATA) LARVAE.JOURNAL OF SHELLFISH RESEARCH,27(5),1125-1128. |
| MLA | Liu, Baozhong,et al."PRELIMINARY STUDIES ON CRYOPRESERVATION OF SYDNEY ROCK OYSTER (SACCOSTREA GLOMERATA) LARVAE".JOURNAL OF SHELLFISH RESEARCH 27.5(2008):1125-1128. |
入库方式： OAI收割
来源：海洋研究所
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