Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies
文献类型：期刊论文
| 作者 | Xu Peng ; Tan Xun-Gang; Zhang Pei-Jun; Zhang Yu-Qing; Wang Bo
|
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 34期号:11页码:1169-1174 |
| 关键词 | Flounder Expression Purification Mrf4 Antibody |
| ISSN号 | 1000-3282 |
| 文献子类 | Article |
| 英文摘要 | MRF4 is one of muscle regulatory factors and plays critical roles during skeletal muscle development. The muscle development is important for the fish growth which is an important economic factor for the fish culture. To analyze the function of MRF4 in fish, the founder MRF4 antibody was prepared. The flounder MRF4 was cloned, ligated into prokaryotic expression vector pET-30b and expressed in strain E. coli BL21 (130). The recombinant flounder MRF4 fusion protein was soluble and purified with cobalt IMAC resins. To prepare MRF4 polyclonal antibodies, rabbits were immunized with the soluble protein and the increasing level of antibodies was determined by Western blot. Also, the endogenous flounder MRF4 was recognized by the anti-serum. The result further proved the existence of the anti-MRF4 antibody in the anti-serum, which will be useful for studies on the function of flounder MRF4.; MRF4 is one of muscle regulatory factors and plays critical roles during skeletal muscle development. The muscle development is important for the fish growth which is an important economic factor for the fish culture. To analyze the function of MRF4 in fish, the founder MRF4 antibody was prepared. The flounder MRF4 was cloned, ligated into prokaryotic expression vector pET-30b and expressed in strain E. coli BL21 (130). The recombinant flounder MRF4 fusion protein was soluble and purified with cobalt IMAC resins. To prepare MRF4 polyclonal antibodies, rabbits were immunized with the soluble protein and the increasing level of antibodies was determined by Western blot. Also, the endogenous flounder MRF4 was recognized by the anti-serum. The result further proved the existence of the anti-MRF4 antibody in the anti-serum, which will be useful for studies on the function of flounder MRF4. |
| 语种 | 英语 |
| WOS记录号 | WOS:000251022800008 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6305]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu Peng,Tan Xun-Gang,Zhang Pei-Jun,et al. Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(11):1169-1174. |
| APA | Xu Peng,Tan Xun-Gang,Zhang Pei-Jun,Zhang Yu-Qing,&Wang Bo.(2007).Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(11),1169-1174. |
| MLA | Xu Peng,et al."Expression, purification of recombinant flounder MRF4 protein in Escherichia coli and analysis of its polyclonal antibodies".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.11(2007):1169-1174. |
入库方式： OAI收割
来源：海洋研究所
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