Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus
文献类型：期刊论文
| 作者 | You, Feng; Xu, Jianhe; Zhu, Xiangping; Xu, Yongli; Zhang, Peijun |
| 刊名 | JOURNAL OF THE WORLD AQUACULTURE SOCIETY
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 39期号:3页码:414-422 |
| 关键词 | Gynogenesis Induction Spermatozoa Ultrastructure Manipulation Hirame Motility Fishes |
| ISSN号 | 0893-8849 |
| 文献子类 | Article |
| 英文摘要 | In this study, at proper dosage of ultraviolet (UV) irradiation (180 sec: 36,000 erg/mm(2)), sperm chromosomes of left-eyed flounder, Paralichthys olivaceus, were inactivated, while spermatozoa maintained ability to move and inseminate eggs. Gynogenetic haploids were detected by morphological observation, chromosome counting, and flow cytometer analysis. The ultrastructure of treated sperm was observed under scanning electronic microscope (SEM) and transmission electronic microscope (TEM). The results showed that after being irradiated at lower dosage of irradiation (0-180 sec: 0-36,000 erg/mm(2)), the surface structure of spermatozoa was not affected by UV irradiation, while the inner structures including membrane system and karyoplasm denseness of treated spermatozoa were little changed. However, obvious changes were observed in their membrane system, mitochondria, and nucleus if the dosage of irradiation increased to 240 sec: 48,000 erg/mm(2) or 300 sec: 60,000 erg/mm(2). The sperm survival rates did not change at the lower dosages of the UV irradiation (0-180 sec: 0-36,000 erg/mm(2)) but decreased as the irradiation dosage increased. The motility of treated sperm was lower than that of control group in general but did not change with UV irradiation dosage increasing at the certain range of 0-300 sec: 0-60,000 erg/mm(2).; In this study, at proper dosage of ultraviolet (UV) irradiation (180 sec: 36,000 erg/mm(2)), sperm chromosomes of left-eyed flounder, Paralichthys olivaceus, were inactivated, while spermatozoa maintained ability to move and inseminate eggs. Gynogenetic haploids were detected by morphological observation, chromosome counting, and flow cytometer analysis. The ultrastructure of treated sperm was observed under scanning electronic microscope (SEM) and transmission electronic microscope (TEM). The results showed that after being irradiated at lower dosage of irradiation (0-180 sec: 0-36,000 erg/mm(2)), the surface structure of spermatozoa was not affected by UV irradiation, while the inner structures including membrane system and karyoplasm denseness of treated spermatozoa were little changed. However, obvious changes were observed in their membrane system, mitochondria, and nucleus if the dosage of irradiation increased to 240 sec: 48,000 erg/mm(2) or 300 sec: 60,000 erg/mm(2). The sperm survival rates did not change at the lower dosages of the UV irradiation (0-180 sec: 0-36,000 erg/mm(2)) but decreased as the irradiation dosage increased. The motility of treated sperm was lower than that of control group in general but did not change with UV irradiation dosage increasing at the certain range of 0-300 sec: 0-60,000 erg/mm(2). |
| 语种 | 英语 |
| WOS记录号 | WOS:000256110300011 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6307]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Feng,Xu, Jianhe,Zhu, Xiangping,et al. Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2008,39(3):414-422. |
| APA | You, Feng,Xu, Jianhe,Zhu, Xiangping,Xu, Yongli,&Zhang, Peijun.(2008).Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,39(3),414-422. |
| MLA | You, Feng,et al."Effect of ultraviolet irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 39.3(2008):414-422. |
入库方式： OAI收割
来源：海洋研究所
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