A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)
文献类型：期刊论文
| 作者 | Hu, Yong-Hua; Sun, Li |
| 刊名 | VACCINE
 |
| 出版日期 | 2011-06-10 |
| 卷号 | 29期号:26页码:4328-4333 |
| 关键词 | Dna Vaccine Vibrio Harveyi Cross Protection Immune Gene |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2011.04.021 |
| 文献子类 | Article |
| 英文摘要 | Vibrio harveyi is a severe aquaculture pathogen that can infect a large number of fish species. In previous studies, we have identified two protective immunogens, DegQ and Vhp1, from pathogenic V. harveyi strains. In this study, we examined the potentials of DegQ and Vhp1 as monovalent and bivalent DNA vaccines in a Japanese flounder (Paralichthys olivaceus) model. For this purpose, the DNA vaccine plasmids pDegQ pVhp1, and pDV were constructed. pDegQ and pVhp1 express, respectively, degQ and vhp1, while pDV expresses degQ and v hp 1 as a bicistronic mRNA transcript (degQ-vhp1). Following vaccination, all vaccine plasmids were detected in muscle, spleen, and kidney at 7 and 14 days post-vaccination. At the same time points and in the same tissues, degQ, vhp1, and degQ-vhp1 mRNA transcripts were detected in, respectively, pDegQ-, pVhp1-, and pDV-vaccinated fish. Production of DegQ and Vhp1 proteins was found in the muscle tissues of pDegQ- and pVhp1-vaccinated fish respectively, while both DegQ and Vhp1 proteins were detected in pDV-vaccinated fish. Compared to control fish, fish vaccinated with pDegQ and pVhp1 exhibited, respectively, 64.1% and 56.5% relative percent survival rates following lethal V. harveyi challenge, while fish vaccinated with pDV exhibited a survival rate that is more than 20% higher than those induced by pDegQ and pVhp1. Furthermore, in addition to V. harveyi, pDV also elicited dramatic cross protection against Vibrio parahaemolyticus. Immunological analysis showed that all vaccine plasmids induced the production of specific serum antibodies and the expression of a wide range of immune genes; however, the induction folds effected by pDV were in general higher than those effected by pDegQ and pVhp1. These results indicate that DNA vaccines, such as that represented by pDV, is a good vaccination option to combat V. harveyi-related vibriosis. (C) 2011 Elsevier Ltd. All rights reserved.; Vibrio harveyi is a severe aquaculture pathogen that can infect a large number of fish species. In previous studies, we have identified two protective immunogens, DegQ and Vhp1, from pathogenic V. harveyi strains. In this study, we examined the potentials of DegQ and Vhp1 as monovalent and bivalent DNA vaccines in a Japanese flounder (Paralichthys olivaceus) model. For this purpose, the DNA vaccine plasmids pDegQ pVhp1, and pDV were constructed. pDegQ and pVhp1 express, respectively, degQ and vhp1, while pDV expresses degQ and v hp 1 as a bicistronic mRNA transcript (degQ-vhp1). Following vaccination, all vaccine plasmids were detected in muscle, spleen, and kidney at 7 and 14 days post-vaccination. At the same time points and in the same tissues, degQ, vhp1, and degQ-vhp1 mRNA transcripts were detected in, respectively, pDegQ-, pVhp1-, and pDV-vaccinated fish. Production of DegQ and Vhp1 proteins was found in the muscle tissues of pDegQ- and pVhp1-vaccinated fish respectively, while both DegQ and Vhp1 proteins were detected in pDV-vaccinated fish. Compared to control fish, fish vaccinated with pDegQ and pVhp1 exhibited, respectively, 64.1% and 56.5% relative percent survival rates following lethal V. harveyi challenge, while fish vaccinated with pDV exhibited a survival rate that is more than 20% higher than those induced by pDegQ and pVhp1. Furthermore, in addition to V. harveyi, pDV also elicited dramatic cross protection against Vibrio parahaemolyticus. Immunological analysis showed that all vaccine plasmids induced the production of specific serum antibodies and the expression of a wide range of immune genes; however, the induction folds effected by pDV were in general higher than those effected by pDegQ and pVhp1. These results indicate that DNA vaccines, such as that represented by pDV, is a good vaccination option to combat V. harveyi-related vibriosis. (C) 2011 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Research & Experimental Medicine |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000292353600006 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11740]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yong-Hua,Sun, Li. A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)[J]. VACCINE,2011,29(26):4328-4333. |
| APA | Hu, Yong-Hua,&Sun, Li.(2011).A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus).VACCINE,29(26),4328-4333. |
| MLA | Hu, Yong-Hua,et al."A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)".VACCINE 29.26(2011):4328-4333. |
入库方式： OAI收割
来源：海洋研究所
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