A macrophage migration inhibitory factor like gene from scallop Chlamys farreri: Involvement in immune response and wound healing
文献类型：期刊论文
| 作者 | Li, Fengmei1,2; Huang, Shuyan2; Wang, Lingling1; Yang, Jialong1; Zhang, Huan1; Qiu, Limei1; Li, Ling1; Song, Linsheng1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 35期号:1页码:62-71 |
| 关键词 | Macrophage Migration Inhibitory Factor Chlamys Farreri Mrna Expression Immune Response Fibroblast Migration |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2010.08.009 |
| 文献子类 | Article |
| 英文摘要 | Macrophage migration inhibitory factor (MIF) is an evolutionarily ancient and highly conserved cytokine with multiple functions. In the present study, a MIF-like gene was cloned from Zhikong scallop Chlamys farreri (designated as CfMIF) based on expressed sequence tag (EST) analysis and rapid amplification of cDNA ends (RACE) approach. The full-length cDNA of CfMIF was of 2296 bp, consisting of a 5' untranslated region (UTR) of 60 bp, a 3' UTR of 1903 bp with a poly(A) tail and an open reading frame (ORF) of 333 bp encoded 111 amino acid residues with a calculated molecular mass of 12.6 kDa and a theoretical iso-electric point of 5.63. The deduced amino acid sequence of CfMIF shared 27-50.5% similarity with those of other known MIFs. A conserved MIF domain was identified in the deduced amino acid sequence of CfMIF, and conserved proline(2) and lysine(33) were also found to be present in CfMIF. Phylogenetic analysis revealed that CfMIF is one of MIF members. The tissue distribution and temporal expression of CfMIF in hemocytes of scallop after lipopolysaccharide (LPS), peptidoglycan (PGN) and beta-glucan stimulation were detected by real-time RT-PCR. CfMIF gene was ubiquitously expressed in six selected tissues of healthy scallops, with the higher expression levels in hepatopancreas, mantle and gill. In comparison with the control group, the expression of CfMIF mRNA in hemocytes was up-regulated significantly at 6h, 24h and 48 h after LPS treatment, and at all time points after PGN and glucan treatment. The cDNA fragment encoding mature peptide of CfMIF was recombined and expressed in Escherichia coli BL21 (DE3) pLysS. The recombinant protein of CfMIF (rCfMIF) promoted sheep fibroblast migration into scraped spaces in vitro. These results generated from the present study encourage us to suggest that CfMIF was a novel member of MIF family, and it was involved in immune response and wound healing by promoting fibroblast migration. (C) 2010 Elsevier Ltd. All rights reserved.; Macrophage migration inhibitory factor (MIF) is an evolutionarily ancient and highly conserved cytokine with multiple functions. In the present study, a MIF-like gene was cloned from Zhikong scallop Chlamys farreri (designated as CfMIF) based on expressed sequence tag (EST) analysis and rapid amplification of cDNA ends (RACE) approach. The full-length cDNA of CfMIF was of 2296 bp, consisting of a 5' untranslated region (UTR) of 60 bp, a 3' UTR of 1903 bp with a poly(A) tail and an open reading frame (ORF) of 333 bp encoded 111 amino acid residues with a calculated molecular mass of 12.6 kDa and a theoretical iso-electric point of 5.63. The deduced amino acid sequence of CfMIF shared 27-50.5% similarity with those of other known MIFs. A conserved MIF domain was identified in the deduced amino acid sequence of CfMIF, and conserved proline(2) and lysine(33) were also found to be present in CfMIF. Phylogenetic analysis revealed that CfMIF is one of MIF members. The tissue distribution and temporal expression of CfMIF in hemocytes of scallop after lipopolysaccharide (LPS), peptidoglycan (PGN) and beta-glucan stimulation were detected by real-time RT-PCR. CfMIF gene was ubiquitously expressed in six selected tissues of healthy scallops, with the higher expression levels in hepatopancreas, mantle and gill. In comparison with the control group, the expression of CfMIF mRNA in hemocytes was up-regulated significantly at 6h, 24h and 48 h after LPS treatment, and at all time points after PGN and glucan treatment. The cDNA fragment encoding mature peptide of CfMIF was recombined and expressed in Escherichia coil BL21 (DE3) pLysS. The recombinant protein of CfMIF (rCfMIF) promoted sheep fibroblast migration into scraped spaces in vitro. These results generated from the present study encourage us to suggest that CfMIF was a novel member of MIF family, and it was involved in immune response and wound healing by promoting fibroblast migration. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000285024400008 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11748]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Fengmei,Huang, Shuyan,Wang, Lingling,et al. A macrophage migration inhibitory factor like gene from scallop Chlamys farreri: Involvement in immune response and wound healing[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(1):62-71. |
| APA | Li, Fengmei.,Huang, Shuyan.,Wang, Lingling.,Yang, Jialong.,Zhang, Huan.,...&Song, Linsheng.(2011).A macrophage migration inhibitory factor like gene from scallop Chlamys farreri: Involvement in immune response and wound healing.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(1),62-71. |
| MLA | Li, Fengmei,et al."A macrophage migration inhibitory factor like gene from scallop Chlamys farreri: Involvement in immune response and wound healing".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.1(2011):62-71. |
入库方式： OAI收割
来源：海洋研究所
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