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A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis

文献类型：期刊论文

作者Mu, Changkao1,2; Zheng, Peilin1; Zhao, Jianmin1; Wang, Lingling1; Qiu, Limei1; Zhang, Huan1; Gai, Yunchao1; Song, Linsheng1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2011-07-01
卷号31期号:1页码:142-147
关键词Eriocheir Sinensis Crustin Bacteria Challenge Antibacterial Activity Minimal Inhibitory Concentration (Mic)
ISSN号1050-4648
DOI10.1016/j.fsi.2011.04.013
文献子类Article
英文摘要Antimicrobial peptides are important effectors in the host innate immune response against microbial invasion. In the present study, the cDNA encoding a crustin (designated CrusEs2) was cloned from Chinese mitten crab Eriocheir sinensis by using EST analysis and rapid amplification of cDNA ends (RACE) approach. The full-length cDNA of CrusEs2 was of 1237 bp, containing a 5' untranslated region (UTR) of 12 bp, a 3' UTR of 886 bp with a poly (A) tail, and an open reading frame (ORF) of 339 bp encoding a polypeptide of 112 amino acids with a signal peptide of 19 amino acids. The CrusEs2 contained a typical WAP domain, but lacked the Gly-rich domain of the type II crustin and the Cys-rich region present in both type I and type II crustin, suggesting that CrusEs2 should be classified as a type III crustin. The mRNA transcripts of CrusEs2 could be detected in haemocytes and gill, and its expression level in haemocytes was up-regulated after Listonella anguillarum challenge, while decreased after Micrococcus luteus challenge. The mature peptide coding region of CrusEs2 was cloned into pET-21a (+) and expressed in Escherichia coli. The purified recombinant CrusEs2 inhibited the growth of Gram-positive bacteria at MIC of 0.093-0.37 mu M. The results indicated that CrusEs2 was involved in immune response of E. sinensis against bacterial challenge. (C) 2011 Elsevier Ltd. All rights reserved.; Antimicrobial peptides are important effectors in the host innate immune response against microbial invasion. In the present study, the cDNA encoding a crustin (designated CrusEs2) was cloned from Chinese mitten crab Eriocheir sinensis by using EST analysis and rapid amplification of cDNA ends (RACE) approach. The full-length cDNA of CrusEs2 was of 1237 bp, containing a 5' untranslated region (UTR) of 12 bp, a 3' UTR of 886 bp with a poly (A) tail, and an open reading frame (ORF) of 339 bp encoding a polypeptide of 112 amino acids with a signal peptide of 19 amino acids. The CrusEs2 contained a typical WAP domain, but lacked the Gly-rich domain of the type II crustin and the Cys-rich region present in both type I and type II crustin, suggesting that CrusEs2 should be classified as a type III crustin. The mRNA transcripts of CrusEs2 could be detected in haemocytes and gill, and its expression level in haemocytes was up-regulated after Listonella anguillarum challenge, while decreased after Micrococcus luteus challenge. The mature peptide coding region of CrusEs2 was cloned into pET-21a (+) and expressed in Escherichia coli. The purified recombinant CrusEs2 inhibited the growth of Gram-positive bacteria at MIC of 0.093-0.37 mu M. The results indicated that CrusEs2 was involved in immune response of E. sinensis against bacterial challenge. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识查看原文
语种英语
WOS记录号WOS:000292407900017
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11760]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China
推荐引用方式
GB/T 7714		 Mu, Changkao,Zheng, Peilin,Zhao, Jianmin,et al. A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(1):142-147.
APA Mu, Changkao.,Zheng, Peilin.,Zhao, Jianmin.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2011).A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,31(1),142-147.
MLA Mu, Changkao,et al."A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 31.1(2011):142-147.

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来源：海洋研究所

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