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AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain

文献类型：期刊论文

作者Zhang, Huan1,2; Song, Xiaoyan1; Wang, Lingling1; Kong, Pengfei1,2; Yang, Jialong1,2; Liu, Lin1,2; Qiu, Limei1; Zhang, Ying1,2; Qiu, Lihua3; Song, Linsheng1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2011
卷号30期号:1页码:17-26
关键词Argopecten Irradians C-type Lectin Innate Immunity Gene Cloning Real-time Pcr Recombinant Expression Bacterial Agglutination
ISSN号1050-4648
DOI10.1016/j.fsi.2009.12.019
文献子类Article
英文摘要C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, a novel C-type lectin gene from scallop Argopecten irradians (designated as AiCTL-6) was cloned by rapid amplification of cDNA ends (RACE) approach based on expression sequence tag (EST) analysis. The full-length cDNA of AiCTL-6 was 1080 bp. The open reading frame encoded a polypeptide of 307 amino acids, including a signal sequence and a C-type lectin-like domain (CTLD) of 150 amino acid residues longer than any usual CTLD. It contained six conserved cysteine residues involved in the formation of three internal disulfide bridges and an EPD (Glu(269)-Pro(270)-Asp(271)) motif at the Ca(2+)-binding site 2. The deduced amino acid sequence of AiCTL-6 showed high similarity to members of C-type lectin superfamily. By fluorescent quantitative real-time PCR, AiCTL-6 mRNA was found mainly in hepatopancreas and gill, and marginally expressed in other tissues. After the scallops were challenged by Listonella anguillarum for 6 h, the mRNA expression of AiCTL-6 was up-regulated significantly to 7.2-fold compared to the blank group. While at 9 h post Micrococcus luteus challenge, its expression level was 60.1 times higher than that of the blank group. The functional activity of AiCTL-6 was investigated by recombination and expression of the cDNA fragment encoding its mature peptide in Escherichia coli Rosetta gami (DE3). The recombinant AiCTL-6 could agglutinate Gram-negative bacteria E. coli TOP10F', Gram-positive bacteria M. luteus and Staphylococcus aureus. These results collectively suggested that AiCTL-6, as a novel member of C-type lectin family, contributed to the host defense mechanisms against invading microorganism in A. irradians. (C) 2009 Elsevier Ltd. All rights reserved.; C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, a novel C-type lectin gene from scallop Argopecten irradians (designated as AiCTL-6) was cloned by rapid amplification of cDNA ends (RACE) approach based on expression sequence tag (EST) analysis. The full-length cDNA of AiCTL-6 was 1080 bp. The open reading frame encoded a polypeptide of 307 amino acids, including a signal sequence and a C-type lectin-like domain (CTLD) of 150 amino acid residues longer than any usual CTLD. It contained six conserved cysteine residues involved in the formation of three internal disulfide bridges and an EPD (Glu(269)-Pro(270)-Asp(271)) motif at the Ca(2+)-binding site 2. The deduced amino acid sequence of AiCTL-6 showed high similarity to members of C-type lectin superfamily. By fluorescent quantitative real-time PCR, AiCTL-6 mRNA was found mainly in hepatopancreas and gill, and marginally expressed in other tissues. After the scallops were challenged by Listonella anguillarum for 6 h, the mRNA expression of AiCTL-6 was up-regulated significantly to 7.2-fold compared to the blank group. While at 9 h post Micrococcus luteus challenge, its expression level was 60.1 times higher than that of the blank group. The functional activity of AiCTL-6 was investigated by recombination and expression of the cDNA fragment encoding its mature peptide in Escherichia coli Rosetta gami (DE3). The recombinant AiCTL-6 could agglutinate Gram-negative bacteria E. coli TOP10F', Gram-positive bacteria M. luteus and Staphylococcus aureus. These results collectively suggested that AiCTL-6, as a novel member of C-type lectin family, contributed to the host defense mechanisms against invading microorganism in A. irradians. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识查看原文
语种英语
WOS记录号WOS:000286905800002
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11770]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Huan,Song, Xiaoyan,Wang, Lingling,et al. AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):17-26.
APA Zhang, Huan.,Song, Xiaoyan.,Wang, Lingling.,Kong, Pengfei.,Yang, Jialong.,...&Song, Linsheng.(2011).AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain.FISH & SHELLFISH IMMUNOLOGY,30(1),17-26.
MLA Zhang, Huan,et al."AiCTL-6, a novel C-type lectin from bay scallop Argopecten irradians with a long C-type lectin-like domain".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):17-26.

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来源：海洋研究所

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