中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
An effective method for parentage determination of the clam (Meretrix meretrix) based on SSR and COI markers

文献类型:期刊论文

作者Lu, Xia1,2; Wang, Hongxia1; Liu, Baozhong1; Xiang, Jianhai1
刊名AQUACULTURE
出版日期2011-07-27
卷号318期号:1-2页码:223-228
关键词Microsatellite Marker Mitochondrial Cytochrome Oxidase (Coi) Meretrix Meretrix Parentage Determination
ISSN号0044-8486
DOI10.1016/j.aquaculture.2011.05.017
文献子类Article
英文摘要Because of the high frequencies of null alleles at microsatellite loci in mollusks, it is difficult to enhance the accuracy of parentage assignment in closely related families by increasing the number of loci. To develop an effective approach, microsatellite markers combined with mitochondrion COI marker were used for parentage assignment with computer simulations and experimental verification in the clam, Meretrix meretrix. In the present study, simulations based on allele frequency data from the candidate parents (sample II) demonstrated that combined exclusion probability of the five microsatellite loci (0.991 for Excl 1 and 0.995 for Excl 2) were not much lower than that of the seven loci (0.993 and 0.999). After discarding the two loci that deviated from Hardy-Weinberg equilibrium in experimental verification, accurate probability of progeny assigned to their fathers and mothers with both parents unknown was 68% and 59% in CF (closely related families), and 76% and 68% in DF (distant related families), respectively, which were much lower than those predicted by the simulations. When the COI marker was used in combination with microsatellite markers, 32 out of 39 offspring that were not previously assigned to their correct parents were assigned to their correct mothers, and 29 doubtful offspring could be assigned to their correct fathers. Consequently, with the combination of the two kinds of markers, the accurate probability of progeny that can be correctly assigned to their true fathers and mothers was increased to 89% and 92% respectively. We suggest that the use of microsatellite markers and COI marker will greatly improve the efficiency of parentage determination in closely related families in the clam. (C) 2011 Elsevier B.V. All rights reserved.; Because of the high frequencies of null alleles at microsatellite loci in mollusks, it is difficult to enhance the accuracy of parentage assignment in closely related families by increasing the number of loci. To develop an effective approach, microsatellite markers combined with mitochondrion COI marker were used for parentage assignment with computer simulations and experimental verification in the clam, Meretrix meretrix. In the present study, simulations based on allele frequency data from the candidate parents (sample II) demonstrated that combined exclusion probability of the five microsatellite loci (0.991 for Excl 1 and 0.995 for Excl 2) were not much lower than that of the seven loci (0.993 and 0.999). After discarding the two loci that deviated from Hardy-Weinberg equilibrium in experimental verification, accurate probability of progeny assigned to their fathers and mothers with both parents unknown was 68% and 59% in CF (closely related families), and 76% and 68% in DF (distant related families), respectively, which were much lower than those predicted by the simulations. When the COI marker was used in combination with microsatellite markers, 32 out of 39 offspring that were not previously assigned to their correct parents were assigned to their correct mothers, and 29 doubtful offspring could be assigned to their correct fathers. Consequently, with the combination of the two kinds of markers, the accurate probability of progeny that can be correctly assigned to their true fathers and mothers was increased to 89% and 92% respectively. We suggest that the use of microsatellite markers and COI marker will greatly improve the efficiency of parentage determination in closely related families in the clam. (C) 2011 Elsevier B.V. All rights reserved.
学科主题Fisheries ; Marine & Freshwater Biology
URL标识查看原文
语种英语
WOS记录号WOS:000292616000034
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11776]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Lu, Xia,Wang, Hongxia,Liu, Baozhong,et al. An effective method for parentage determination of the clam (Meretrix meretrix) based on SSR and COI markers[J]. AQUACULTURE,2011,318(1-2):223-228.
APA Lu, Xia,Wang, Hongxia,Liu, Baozhong,&Xiang, Jianhai.(2011).An effective method for parentage determination of the clam (Meretrix meretrix) based on SSR and COI markers.AQUACULTURE,318(1-2),223-228.
MLA Lu, Xia,et al."An effective method for parentage determination of the clam (Meretrix meretrix) based on SSR and COI markers".AQUACULTURE 318.1-2(2011):223-228.

入库方式: OAI收割

来源:海洋研究所

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