An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity
文献类型：期刊论文
| 作者 | Yue, Xin1,2; Liu, Baozhong1 ; Xue, Qinggang3
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 30期号:2页码:550-558 |
| 关键词 | Invertebrate Lysozyme Meretrix Meretrix Recombinant Expression Antimicrobial Activity Immunity |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2010.11.022 |
| 文献子类 | Article |
| 英文摘要 | Lysozymes function in animal immunity. Three types of lysozyme\ have been identified in animal kingdom and most lysozymes identified from bivalve molluscs belong to the invertebrate (i) type. In this research, we cloned and sequenced a new i-type lysozyme, named MmeLys, from the Asiatic hard clam Meretrix meretrix. MmeLys cDNA was constituted of 552 bp, with a 441 bp open reading frame encoding a 146 amino acid polypeptide. The encoded polypeptide was predicted to have a 15 amino acid signal peptide, and a 131 amino acid mature protein with a theoretical mass of 14601.44 Da and an isoelectric point (pl) of 7.14. MmeLys amino acid sequence bore 64% identity with the Manila clam (Venerupis philippinarum) i-type lysozyme and was grouped with other veneroid i-type lysozymes in a bivalve lysozyme phylogenetic tree predicted using Neighbor-Jointing method. Recombinantly expressed MmeLys showed lysozyme activity and strong antibacterial activity against Gram positive and Gram negative bacteria. MmeLys mRNA and protein were detected to be mainly produced in hepatopancreas and gill by the methods of semi-quantitative RT-PCR and western blotting. In addition, MmeLys gene expression increased following Vibrio parahaemolyticus challenge. Results of this research indicated that MmeLys represents a new i-type lysozyme that likely functions in M. meretrix immunity. (C) 2010 Elsevier Ltd. All rights reserved.; Lysozymes function in animal immunity. Three types of lysozyme\ have been identified in animal kingdom and most lysozymes identified from bivalve molluscs belong to the invertebrate (i) type. In this research, we cloned and sequenced a new i-type lysozyme, named MmeLys, from the Asiatic hard clam Meretrix meretrix. MmeLys cDNA was constituted of 552 bp, with a 441 bp open reading frame encoding a 146 amino acid polypeptide. The encoded polypeptide was predicted to have a 15 amino acid signal peptide, and a 131 amino acid mature protein with a theoretical mass of 14601.44 Da and an isoelectric point (pl) of 7.14. MmeLys amino acid sequence bore 64% identity with the Manila clam (Venerupis philippinarum) i-type lysozyme and was grouped with other veneroid i-type lysozymes in a bivalve lysozyme phylogenetic tree predicted using Neighbor-Jointing method. Recombinantly expressed MmeLys showed lysozyme activity and strong antibacterial activity against Gram positive and Gram negative bacteria. MmeLys mRNA and protein were detected to be mainly produced in hepatopancreas and gill by the methods of semi-quantitative RT-PCR and western blotting. In addition, MmeLys gene expression increased following Vibrio parahaemolyticus challenge. Results of this research indicated that MmeLys represents a new i-type lysozyme that likely functions in M. meretrix immunity. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000288305300014 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11782]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Louisiana State Univ, Dept Vet Sci, Ctr Agr, Baton Rouge, LA 70803 USA |
| 推荐引用方式 GB/T 7714 | Yue, Xin,Liu, Baozhong,Xue, Qinggang. An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(2):550-558. |
| APA | Yue, Xin,Liu, Baozhong,&Xue, Qinggang.(2011).An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity.FISH & SHELLFISH IMMUNOLOGY,30(2),550-558. |
| MLA | Yue, Xin,et al."An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity".FISH & SHELLFISH IMMUNOLOGY 30.2(2011):550-558. |
入库方式： OAI收割
来源：海洋研究所
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