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Biosynthesis and Immobilization of Biofunctional Allophycocyanin

文献类型：期刊论文

作者Chen, Yingjie1,2; Liu, Shaofang1,2; Cui, Yulin1,2; Jiang, Peng1; Chen, Huaxin1; Li, Fuchao1; Qin, Song1
刊名EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
出版日期2011
页码751452
ISSN号1741-427X
DOI10.1155/2011/751452
文献子类Article
英文摘要The holo-allophycocyanin-a subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles. The holo-allophycocyanin-a subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles. Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.; The holo-allophycocyanin-a subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles. The holo-allophycocyanin-a subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles. Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.
学科主题Integrative & Complementary Medicine
URL标识查看原文
语种英语
WOS记录号WOS:000295672500001
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11788]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Yingjie,Liu, Shaofang,Cui, Yulin,et al. Biosynthesis and Immobilization of Biofunctional Allophycocyanin[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2011:751452.
APA Chen, Yingjie.,Liu, Shaofang.,Cui, Yulin.,Jiang, Peng.,Chen, Huaxin.,...&Qin, Song.(2011).Biosynthesis and Immobilization of Biofunctional Allophycocyanin.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,751452.
MLA Chen, Yingjie,et al."Biosynthesis and Immobilization of Biofunctional Allophycocyanin".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2011):751452.

入库方式： OAI收割

来源：海洋研究所

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