Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda
文献类型：期刊论文
| 作者 | Li, G. Y.1,2; Li, J.1,2 ; Xiao, P.1; Guo, Y. H.3; Mo, Z. L.1
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| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
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| 出版日期 | 2011-03-01 |
| 卷号 | 52期号:3页码:213-219 |
| 关键词 | Edwardsiella Tarda Pathogen Detection T3ss |
| ISSN号 | 0266-8254 |
| DOI | 10.1111/j.1472-765X.2010.02984.x |
| 文献子类 | Article |
| 英文摘要 | Aims:; Aims: To differentiate pathogenic and nonpathogenic Edwardsiella tarda strains based on the detection of type III secretion system (T3SS) gene using polymerase chain reaction (PCR). Methods and Results: Primers were designed to amplify Edw. tarda T3SS component gene esaV, catalase gene katB, haemolysin gene hlyA and 16S rRNA gene as an internal positive control. Genomic DNAs were extracted using a commercial isolation kit from 36 Edw. tarda strains consisting of 18 pathogenic and 18 nonpathogenic strains, and 50 ng of each DNA was used as the template for PCR amplification. PCR was performed with a thermocycler (TaKaRa TP600) in a 25-mu l volume. Products of esaV were detected in all pathogenic strains, but not in nonpathogenic strains; katB was detected in all pathogenic strains and one of nonpathogenic strains; hlyA was not detected in any strains. Conclusions: The detection of esaV gene can be used for the assessment of pathogenic Edw. tarda strains. Significance and Impact of the Study: The strategy using T3SS gene as the virulence indicator provides a useful tool for the clinical assessment of pathogenic Edw. tarda strains and prediction of edwardsiellosis risk in fish culture environments. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287038400005 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11830]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Fujian Key Lab Med Instrument & Pharmaceut Techno, Fuzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, G. Y.,Li, J.,Xiao, P.,et al. Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda[J]. LETTERS IN APPLIED MICROBIOLOGY,2011,52(3):213-219. |
| APA | Li, G. Y.,Li, J.,Xiao, P.,Guo, Y. H.,&Mo, Z. L..(2011).Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda.LETTERS IN APPLIED MICROBIOLOGY,52(3),213-219. |
| MLA | Li, G. Y.,et al."Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda".LETTERS IN APPLIED MICROBIOLOGY 52.3(2011):213-219. |
入库方式： OAI收割
来源：海洋研究所
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