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Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

文献类型：期刊论文

作者Luo Zhan1,2; Zhang Jiquan1; Li Fuhua1; Zhang Xiaojun1; Liu Chengzhang1,2; Xiang Jianhai1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2011-09-01
卷号29期号:5页码:942-951
关键词Litopenaeus Vannamei Innate Immune Pattern Recognition Receptor C-type Lectin Rna Interference (Rnai) Recombinant Expression
ISSN号0254-4059
DOI10.1007/s00343-011-0249-6
文献子类Article
英文摘要Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLec1 was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLec1 transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLec1 resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic "EPN" (Glu-Pro-Ser) or "QPD" (Gln-Pro-Asp) motif, the recombinant LvLec1, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLec1 might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.; Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLec1 was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLec1 transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLec1 resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic "EPN" (Glu-Pro-Ser) or "QPD" (Gln-Pro-Asp) motif, the recombinant LvLec1, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLec1 might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.
学科主题Marine & Freshwater Biology ; Oceanography
URL标识查看原文
语种英语
WOS记录号WOS:000293632500004
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11876]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Luo Zhan,Zhang Jiquan,Li Fuhua,et al. Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(5):942-951.
APA Luo Zhan,Zhang Jiquan,Li Fuhua,Zhang Xiaojun,Liu Chengzhang,&Xiang Jianhai.(2011).Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(5),942-951.
MLA Luo Zhan,et al."Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.5(2011):942-951.

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来源：海洋研究所

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