Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)
文献类型：期刊论文
| 作者 | Wang Jinxia1,2; Shao Kuishuang3; Cheng Bin4; Lu Qinqin4; Zhou Baicheng1 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 29期号:6页码:1260-1266 |
| 关键词 | Polysiphonia Urceolata Rhodophyta Filament Culture |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-011-0295-0 |
| 文献子类 | Article |
| 英文摘要 | A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22 degrees C and 95-120 mu E/(m(2).s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 mu E/(m(2).s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.; A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22 degrees C and 95-120 mu E/(m(2).s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 mu E/(m(2).s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products. |
| 学科主题 | Marine & Freshwater Biology ; Oceanography |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000299039400013 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11880]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 4.Inst Oceanol & Marine Fisheries, Nantong 226007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Jinxia,Shao Kuishuang,Cheng Bin,et al. Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(6):1260-1266. |
| APA | Wang Jinxia,Shao Kuishuang,Cheng Bin,Lu Qinqin,&Zhou Baicheng.(2011).Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta).CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(6),1260-1266. |
| MLA | Wang Jinxia,et al."Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.6(2011):1260-1266. |
入库方式： OAI收割
来源：海洋研究所
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